Histopathology of nasopharyngeal and palatine tonsils in Japanese black calves naturally infected with Mycoplasma bovis

Mycoplasma bovis (M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The nasopharyngeal and palatine tonsils are mucosal-associated lymphoid tissue components that are the initial barrier to respiratory pathogens. In the pr...

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Published inJournal of Veterinary Medical Science Vol. 87; no. 8; pp. 25-0188 - 933
Main Authors AHMED, Amaal Ezzat, NAKAI, Mutsumi, KAKIYA, Miho, FUKE, Naoyuki, HEGAZY, Asmaa A, KONDO, Hiroaki, HIRAI, Takuya
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2025
The Japanese Society of Veterinary Science
Subjects
Animals
bovine respiratory disease complex
Cattle
Cattle Diseases - microbiology
Cattle Diseases - pathology
In Situ Hybridization - veterinary
Lung - microbiology
Lung - pathology
Male
Mycoplasma bovis
Mycoplasma bovis - isolation & purification
Mycoplasma Infections - microbiology
Mycoplasma Infections - pathology
Mycoplasma Infections - veterinary
nasopharyngeal tonsil
Nasopharynx - microbiology
Nasopharynx - pathology
palatine tonsil
Palatine Tonsil - microbiology
Palatine Tonsil - pathology
Pathology
Polymerase Chain Reaction - veterinary
cattle
bovine respiratory disease complex
nasopharyngeal tonsil
Mycoplasma bovis
palatine tonsil
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Abstract Mycoplasma bovis (M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The nasopharyngeal and palatine tonsils are mucosal-associated lymphoid tissue components that are the initial barrier to respiratory pathogens. In the present study, we investigated 5 pneumonic Japanese black and 3 control calves (2 Japanese black and 1 Holstein). The localization of M. bovis in the nasopharyngeal, palatine tonsils, and lungs was examined using nested and multiplex polymerase chain reaction (PCR), histopathology, and in situ hybridization (ISH) techniques. Nested PCR revealed that all examined tissues from all pneumonic calves were positive for M. bovis infection, but not the control. Histopathology revealed suppurative nasopharyngeal tonsillitis and palatine tonsillar cryptitis in all cases. Surprisingly, prominent histologic changes were observed in the palatine tonsils but not in the nasopharyngeal tonsils. Necrotizing suppurative bronchopneumonia was observed in 4 pneumonic calves. In ISH analysis, positive hybridization signals adherent to and/or within the surface epithelium of nasopharyngeal tonsils and crypt epithelium of nasopharyngeal and palatine tonsils were observed. Additionally, these signals were seen in the palatine tonsillar cryptic detritus. In the lungs, ISH signals were observed in necrotic areas, the bronchial epithelium, and pulmonary inflammatory exudate. These findings suggest that the nasopharyngeal and palatine tonsils are the primary sites of M. bovis infection. Also, M. bovis can colonize the detritus in the crypts of the palatine tonsils of the pneumonic animals.
AbstractList Mycoplasma bovis (M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The nasopharyngeal and palatine tonsils are mucosal-associated lymphoid tissue components that are the initial barrier to respiratory pathogens. In the present study, we investigated 5 pneumonic Japanese black and 3 control calves (2 Japanese black and 1 Holstein). The localization of M. bovis in the nasopharyngeal, palatine tonsils, and lungs was examined using nested and multiplex polymerase chain reaction (PCR), histopathology, and in situ hybridization (ISH) techniques. Nested PCR revealed that all examined tissues from all pneumonic calves were positive for M. bovis infection, but not the control. Histopathology revealed suppurative nasopharyngeal tonsillitis and palatine tonsillar cryptitis in all cases. Surprisingly, prominent histologic changes were observed in the palatine tonsils but not in the nasopharyngeal tonsils. Necrotizing suppurative bronchopneumonia was observed in 4 pneumonic calves. In ISH analysis, positive hybridization signals adherent to and/or within the surface epithelium of nasopharyngeal tonsils and crypt epithelium of nasopharyngeal and palatine tonsils were observed. Additionally, these signals were seen in the palatine tonsillar cryptic detritus. In the lungs, ISH signals were observed in necrotic areas, the bronchial epithelium, and pulmonary inflammatory exudate. These findings suggest that the nasopharyngeal and palatine tonsils are the primary sites of M. bovis infection. Also, M. bovis can colonize the detritus in the crypts of the palatine tonsils of the pneumonic animals.
Mycoplasma bovis (M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The nasopharyngeal and palatine tonsils are mucosal-associated lymphoid tissue components that are the initial barrier to respiratory pathogens. In the present study, we investigated 5 pneumonic Japanese black and 3 control calves (2 Japanese black and 1 Holstein). The localization of M. bovis in the nasopharyngeal, palatine tonsils, and lungs was examined using nested and multiplex polymerase chain reaction (PCR), histopathology, and in situ hybridization (ISH) techniques. Nested PCR revealed that all examined tissues from all pneumonic calves were positive for M. bovis infection, but not the control. Histopathology revealed suppurative nasopharyngeal tonsillitis and palatine tonsillar cryptitis in all cases. Surprisingly, prominent histologic changes were observed in the palatine tonsils but not in the nasopharyngeal tonsils. Necrotizing suppurative bronchopneumonia was observed in 4 pneumonic calves. In ISH analysis, positive hybridization signals adherent to and/or within the surface epithelium of nasopharyngeal tonsils and crypt epithelium of nasopharyngeal and palatine tonsils were observed. Additionally, these signals were seen in the palatine tonsillar cryptic detritus. In the lungs, ISH signals were observed in necrotic areas, the bronchial epithelium, and pulmonary inflammatory exudate. These findings suggest that the nasopharyngeal and palatine tonsils are the primary sites of M. bovis infection. Also, M. bovis can colonize the detritus in the crypts of the palatine tonsils of the pneumonic animals.Mycoplasma bovis (M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The nasopharyngeal and palatine tonsils are mucosal-associated lymphoid tissue components that are the initial barrier to respiratory pathogens. In the present study, we investigated 5 pneumonic Japanese black and 3 control calves (2 Japanese black and 1 Holstein). The localization of M. bovis in the nasopharyngeal, palatine tonsils, and lungs was examined using nested and multiplex polymerase chain reaction (PCR), histopathology, and in situ hybridization (ISH) techniques. Nested PCR revealed that all examined tissues from all pneumonic calves were positive for M. bovis infection, but not the control. Histopathology revealed suppurative nasopharyngeal tonsillitis and palatine tonsillar cryptitis in all cases. Surprisingly, prominent histologic changes were observed in the palatine tonsils but not in the nasopharyngeal tonsils. Necrotizing suppurative bronchopneumonia was observed in 4 pneumonic calves. In ISH analysis, positive hybridization signals adherent to and/or within the surface epithelium of nasopharyngeal tonsils and crypt epithelium of nasopharyngeal and palatine tonsils were observed. Additionally, these signals were seen in the palatine tonsillar cryptic detritus. In the lungs, ISH signals were observed in necrotic areas, the bronchial epithelium, and pulmonary inflammatory exudate. These findings suggest that the nasopharyngeal and palatine tonsils are the primary sites of M. bovis infection. Also, M. bovis can colonize the detritus in the crypts of the palatine tonsils of the pneumonic animals.
Mycoplasma bovis ( M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The nasopharyngeal and palatine tonsils are mucosal-associated lymphoid tissue components that are the initial barrier to respiratory pathogens. In the present study, we investigated 5 pneumonic Japanese black and 3 control calves (2 Japanese black and 1 Holstein). The localization of M. bovis in the nasopharyngeal, palatine tonsils, and lungs was examined using nested and multiplex polymerase chain reaction (PCR), histopathology, and in situ hybridization (ISH) techniques. Nested PCR revealed that all examined tissues from all pneumonic calves were positive for M. bovis infection, but not the control. Histopathology revealed suppurative nasopharyngeal tonsillitis and palatine tonsillar cryptitis in all cases. Surprisingly, prominent histologic changes were observed in the palatine tonsils but not in the nasopharyngeal tonsils. Necrotizing suppurative bronchopneumonia was observed in 4 pneumonic calves. In ISH analysis, positive hybridization signals adherent to and/or within the surface epithelium of nasopharyngeal tonsils and crypt epithelium of nasopharyngeal and palatine tonsils were observed. Additionally, these signals were seen in the palatine tonsillar cryptic detritus. In the lungs, ISH signals were observed in necrotic areas, the bronchial epithelium, and pulmonary inflammatory exudate. These findings suggest that the nasopharyngeal and palatine tonsils are the primary sites of M. bovis infection. Also, M. bovis can colonize the detritus in the crypts of the palatine tonsils of the pneumonic animals.
ArticleNumber 25-0188
Author AHMED, Amaal Ezzat
HEGAZY, Asmaa A
HIRAI, Takuya
NAKAI, Mutsumi
KONDO, Hiroaki
KAKIYA, Miho
FUKE, Naoyuki
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  fullname: KAKIYA, Miho
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Keywords cattle
bovine respiratory disease complex
nasopharyngeal tonsil
Mycoplasma bovis
palatine tonsil
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Snippet Mycoplasma bovis (M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The...
Mycoplasma bovis ( M. bovis) is an important bacterium in the bovine respiratory disease complex (BRDC), which causes significant economic losses. The...
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SubjectTerms Animals
bovine respiratory disease complex
Cattle
Cattle Diseases - microbiology
Cattle Diseases - pathology
In Situ Hybridization - veterinary
Lung - microbiology
Lung - pathology
Male
Mycoplasma bovis
Mycoplasma bovis - isolation & purification
Mycoplasma Infections - microbiology
Mycoplasma Infections - pathology
Mycoplasma Infections - veterinary
nasopharyngeal tonsil
Nasopharynx - microbiology
Nasopharynx - pathology
palatine tonsil
Palatine Tonsil - microbiology
Palatine Tonsil - pathology
Pathology
Polymerase Chain Reaction - veterinary
Title Histopathology of nasopharyngeal and palatine tonsils in Japanese black calves naturally infected with Mycoplasma bovis
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