Tumor necrosis factor-induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption

• Published in: The FASEB journal Vol. 35; no. 6; p. e21627
• Main Authors: Khan, Alamzeb, Ni, Weiming, Lopez-Giraldez, Francesc, Kluger, Martin S, Pober, Jordan S, Pierce, Richard W
• Format: Journal Article
• Language: English
• Published: United States 01.06.2021
• Subjects: Capillary Permeability; Dermis - cytology; Dermis - drug effects; Dermis - metabolism; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Guanine Nucleotide Exchange Factors - genetics; Guanine Nucleotide Exchange Factors - metabolism; Humans; Rho Guanine Nucleotide Exchange Factors - genetics; Rho Guanine Nucleotide Exchange Factors - metabolism; rhoB GTP-Binding Protein - genetics; rhoB GTP-Binding Protein - metabolism; Signal Transduction; Tight Junctions - physiology; Tumor Necrosis Factor-alpha - pharmacology; Rho guanine nucleotide-exchange factor; capillary leak; vascular dysfunction; inflammation; transendothelial electrical resistance; permeability

Capillary endothelial cells (ECs) maintain a semi-permeable barrier between the blood and tissue by forming inter-EC tight junctions (TJs), regulating selective transport of fluid and solutes. Overwhelming inflammation, as occurs in sepsis, disrupts these TJs, leading to leakage of fluid, proteins,...