The human platelet as a model for the glutamatergic neuron: platelet uptake of L-glutamate

• Published in: Journal of neurochemistry Vol. 36; no. 3; p. 1067
• Main Authors: Mangano, R M, Schwarcz, R
• Format: Journal Article
• Language: English
• Published: England 01.03.1981
• Subjects: Animals; Antimetabolites - pharmacology; Biological Transport - drug effects; Blood Platelets - metabolism; Brain - metabolism; Glutamates - blood; Glutamates - metabolism; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Kinetics; Models, Neurological; Neurons - metabolism; Rats; Sonication; Temperature

L-Glutamate uptake into human platelets revealed two components: a high-affinity system (KmH = 3.1 microM), which was sodium-dependent, and a low-affinity site (Km = 88 microM) displaying temperature rather than sodium dependency. These kinetic properties were similar to those found in crude synapto...