Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes

Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure tar- geting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rho- damine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluoresc...

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Published inBiophysics reports Vol. 3; no. 4; pp. 85 - 91
Main Authors Xie, Tao-Rong, Liu, Chun-Feng, Kang, Jian-Sheng
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 2017
Institute of Biophysics
Subjects
Adipocytes
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Energy
Energy expenditure
Equilibrium
Fluorescence
Life Sciences
Method
Mitochondria
NMR
Norepinephrine
Nuclear magnetic resonance
Open access publishing
Rhodamine
Temperature
Thermogenesis
Thermometry
Nanothermometry
Brown adipocytes
Thermogenesis
Mitochondrial thermometry
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Summary:Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure tar- geting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rho- damine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Using this approach, we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low, and measure the maximum transient rate of tempera- ture increase in brown adipocytes.2010CB912001）, National Natural Science Foundation of China （31171369）, Chinese Academy of Sciences （Hundred Talents Program and 2009OHTP10）.
Bibliography:Mitochondrial thermometry, Nanothermometry, Thermogenesis, Brown adipocytes
10-1302/Q
Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure tar- geting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rho- damine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Using this approach, we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low, and measure the maximum transient rate of tempera- ture increase in brown adipocytes.2010CB912001）, National Natural Science Foundation of China （31171369）, Chinese Academy of Sciences （Hundred Talents Program and 2009OHTP10）.
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ISSN:2364-3439
2364-3420
DOI:10.1007/s41048-017-0039-6