Allele specific LAMP- gold nanoparticle for characterization of single nucleotide polymorphisms

• Published in: Biotechnology reports (Amsterdam, Netherlands) Vol. 16; pp. 21 - 25
• Main Authors: Carlos, Fábio Ferreira, Veigas, Bruno, Matias, Ana S., Doria, Gonçalo, Flores, Orfeu, Baptista, Pedro V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2017; Elsevier
• Subjects: Gold nanoparticles; Gold nanoprobes; Isothermal amplification; Lactose intolerance; SNP; SNP; Lactose intolerance; Isothermal amplification; Gold nanoparticles; Gold nanoprobes

•Allele-specific isothermal amplification method (AS-LAMP) for SNP characterization.•Use of ssDNA-functionalized gold nanoparticles (AuNPs) for SNP full discrimination.•A simple and low-cost strategy to provide fast results in medium throughput settings.•AS-LAMP amplification products can be easily...