Development and evaluation of human T-cell leukemia virus-1 and -2 multiplex quantitative PCR

• Published in: Microbiology and immunology Vol. 63; no. 11; p. 458
• Main Authors: Kuramitsu, Madoka, Okuma, Kazu, Tezuka, Kenta, Nakamura, Hitomi, Sagara, Yasuko, Kurane, Ichiro, Hamaguchi, Isao
• Format: Journal Article
• Language: English
• Published: Australia 01.11.2019
• Subjects: HTLV-I Infections - diagnosis; HTLV-I Infections - virology; HTLV-II Infections - diagnosis; HTLV-II Infections - virology; Human T-lymphotropic virus 1 - genetics; Human T-lymphotropic virus 1 - isolation & purification; Human T-lymphotropic virus 2 - genetics; Human T-lymphotropic virus 2 - isolation & purification; Humans; Japan; Multiplex Polymerase Chain Reaction - methods; Proviruses - genetics; Proviruses - isolation & purification; Real-Time Polymerase Chain Reaction - methods; Sensitivity and Specificity; Japan; diagnosis; HTLV-1; HTLV-2; provirus; quantitative PCR

The diagnosis of human T -cell leukemia virus type 1 (HTLV-1) infection in Japan is usually performed by serological testing, but the high rate of indeterminate results from western blotting makes it difficult to assess the infection accurately. Nucleic acid tests for HTLV-1 and/or HTLV-2 are used t...