Histone Acetylation Level and Histone Acetyltransferase/Deacetylase Activity in Ejaculated Sperm from Normozoospermic Men
The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. Thirty-three normozoospermic men were included in this study. Semen samples were...
Saved in:
| Published in | Yonsei medical journal Vol. 55; no. 5; pp. 1333 - 1340 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Korea (South)
Yonsei University College of Medicine
01.09.2014
연세대학교의과대학 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0513-5796 1976-2437 1976-2437 |
| DOI | 10.3349/ymj.2014.55.5.1333 |
Cover
| Abstract | The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters.
Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commercially available kits. Nuclear DNA integrity was also measured by TUNEL assay.
The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationship with HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r=-0.53, p<0.01).
Our works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI. |
|---|---|
| AbstractList | The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters.PURPOSEThe aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters.Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commercially available kits. Nuclear DNA integrity was also measured by TUNEL assay.MATERIALS AND METHODSThirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commercially available kits. Nuclear DNA integrity was also measured by TUNEL assay.The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationship with HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r=-0.53, p<0.01).RESULTSThe levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationship with HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r=-0.53, p<0.01).Our works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI.CONCLUSIONOur works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI. Purpose: The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. Materials and Methods:Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commerciallyavailable kits. Nuclear DNA integrity was also measured by TUNEL assay. Results: The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationshipwith HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r= -0.53, p<0.01). Conclusion: Our works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI. KCI Citation Count: 14 The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation antibodies (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac). Our preliminary study verified the expression of HAT/HDAC1 in mature human sperm. From vitrified-warmed sperm samples, total HAT/HDAC activity was measured by commercially available kits. Nuclear DNA integrity was also measured by TUNEL assay. The levels of six acetylation marks were not related with conventional sperm parameters including sperm DNA fragmentation index (DFI) as well as HAT/HDAC activity. However, sperm DFI was positively correlated with HAT activity (r=0.038 after adjustment, p<0.02). HAT activity showed a negative relationship with HDAC activity (r=-0.51, p<0.01). Strict morphology was negatively correlated with acetylation enzyme index (=HAT activity/HDAC activity) (r=-0.53, p<0.01). Our works demonstrated a significant relationship of acetylation-associated enzyme activity and strict morphology or sperm DFI. |
| Author | Jee, Byung Chul Kim, Jee Hyun Lee, Jang Mi Suh, Chang Suk Kim, Seok Hyun |
| AuthorAffiliation | 2 Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea 3 Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea 1 Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea |
| AuthorAffiliation_xml | – name: 2 Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea – name: 3 Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea – name: 1 Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea |
| Author_xml | – sequence: 1 givenname: Jee Hyun surname: Kim fullname: Kim, Jee Hyun organization: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea., Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea – sequence: 2 givenname: Byung Chul surname: Jee fullname: Jee, Byung Chul organization: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea., Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea – sequence: 3 givenname: Jang Mi surname: Lee fullname: Lee, Jang Mi organization: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea – sequence: 4 givenname: Chang Suk surname: Suh fullname: Suh, Chang Suk organization: Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea., Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea – sequence: 5 givenname: Seok Hyun surname: Kim fullname: Kim, Seok Hyun organization: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea., Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25048493$$D View this record in MEDLINE/PubMed https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001903947$$DAccess content in National Research Foundation of Korea (NRF) |
| BookMark | eNp9kc1u1DAUhS1URKeFF2CBvGSTqX-TeIM0agutNIAEZW05zg14mtiDnRkpPD3ODBTKgtWVfL5z7rXOGTrxwQNCLylZci7UxTRsloxQsZRyKZeUc_4ELaiqyoIJXp2gBZGUF7JS5Sk6S2lDCKsoYc_QKZNE1ELxBZpuXBpzLF5ZGKfejC54vIY99Nj4Fj9Wx2h86iCaBBdXYI6ONKuj27txws7j642xu5wDLf68hTjgLoYBfwhxCD9CSPOTs_g9-OfoaWf6BC9-zXP05e313eVNsf747vZytS6s4HIsOsO6UnCrJAOuhAVLOShRtZQoUjPZqoY2TSNbWWWNlcAEYTWUxHRNZw3j5-j1MdfHTt9bp4Nxh_k16PuoV5_ubjXnpBIyo2-O6HbXDNBa8PnHvd5GN5g4HYyPFe--5Zi9FpTUNSN_dm1j-L6DNOrBJQt9bzyEXdJUiqpmtWI0o6_-3vWw5Hc1GWBHwMaQUoTuAaFEz_3r3L-e-9dSaqnn_rOp_sdk3XgoNd_r-v9ZfwICqrlt |
| CitedBy_id | crossref_primary_10_1002_slct_202404466 crossref_primary_10_1080_14789450_2019_1693895 crossref_primary_10_3390_jcm9030640 crossref_primary_10_3390_cells11111788 crossref_primary_10_1093_molehr_gaaf003 crossref_primary_10_1016_j_bbagen_2016_04_001 crossref_primary_10_1093_humrep_deae109 crossref_primary_10_1016_j_biocel_2018_02_010 crossref_primary_10_1093_humupd_dmad006 crossref_primary_10_1016_j_fertnstert_2015_03_007 crossref_primary_10_1007_s00412_019_00704_w crossref_primary_10_1038_s41598_020_75758_3 crossref_primary_10_1071_RD22226 crossref_primary_10_1111_and_12800 crossref_primary_10_1186_s13072_021_00433_4 crossref_primary_10_1111_and_13799 crossref_primary_10_1111_and_12814 crossref_primary_10_1007_s11010_021_04116_2 crossref_primary_10_1177_1933719115580997 crossref_primary_10_1038_s41598_018_31557_5 crossref_primary_10_1074_mcp_M114_041384 |
| Cites_doi | 10.1016/j.fertnstert.2008.02.123 10.1016/S0959-437X(98)80138-X 10.1093/humrep/den390 10.1016/S0015-0282(02)04664-2 10.1016/S0074-7696(08)62703-5 10.1007/s10815-012-9715-0 10.1093/humrep/des261 10.1073/pnas.0510946103 10.1042/bj20021321 10.1002/j.1939-4640.2004.tb02859.x 10.1016/j.fertnstert.2009.10.046 10.1016/S1472-6483(10)60454-3 10.1016/S0021-9258(17)30554-9 10.1016/j.ydbio.2008.04.011 10.1046/j.1439-0272.2002.00524.x 10.1038/nrg1654 10.1016/j.tibs.2005.12.008 10.1038/sj.emboj.7601513 10.1111/j.1745-7262.2006.00127.x 10.1016/S1472-6483(10)61798-1 10.1016/S0378-1119(99)00405-9 10.1016/j.fertnstert.2005.03.052 10.1016/j.fertnstert.2012.07.1146 10.1111/j.1439-0272.2005.00656.x 10.1071/RD10197 10.1093/humrep/deh127 10.1078/0171-9335-00123 10.5653/cerm.2011.38.2.82 10.1016/S1472-6483(10)60456-7 10.1242/jcs.99.1.13 10.1038/nature08162 10.1111/j.1365-2605.2010.01129.x 10.1016/S1074-5521(02)00092-3 10.1007/s00418-006-0234-x 10.1006/excr.1996.0188 10.1093/molehr/gag101 |
| ContentType | Journal Article |
| Copyright | Copyright: Yonsei University College of Medicine 2014 2014 |
| Copyright_xml | – notice: Copyright: Yonsei University College of Medicine 2014 2014 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 5PM ACYCR |
| DOI | 10.3349/ymj.2014.55.5.1333 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic PubMed Central (Full Participant titles) Korean Citation Index |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1976-2437 |
| EndPage | 1340 |
| ExternalDocumentID | oai_kci_go_kr_ARTI_330745 PMC4108820 25048493 10_3349_ymj_2014_55_5_1333 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GrantInformation_xml | – fundername: Seoul National University College of Medicine grantid: 800-20110058 |
| GroupedDBID | --- .55 .GJ 123 29R 2WC 36B 5-W 53G 5RE 8JR 8XY 9ZL AAYXX ACYCR ADBBV ADRAZ AEGXH AENEX ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV CITATION CS3 DIK DU5 E3Z EBD EF. EMB EMOBN F5P GROUPED_DOAJ GX1 HYE KQ8 L7B M48 MK0 O5R O5S OK1 OVT P2P PGMZT RNS RPM SV3 TR2 X7M XSB ZGI ZXP CGR CUY CVF ECM EIF NPM 7X8 5PM M~E |
| ID | FETCH-LOGICAL-c435t-fa2f643c952e394cec13e947d1090825d9b1bbb5d57cec26e24028e60afbfca23 |
| IEDL.DBID | M48 |
| ISSN | 0513-5796 1976-2437 |
| IngestDate | Tue Nov 21 21:37:11 EST 2023 Thu Aug 21 18:13:25 EDT 2025 Fri Jul 11 12:44:55 EDT 2025 Thu Apr 03 07:10:55 EDT 2025 Tue Jul 01 04:02:13 EDT 2025 Thu Apr 24 22:56:07 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Keywords | sperm Acetylation deacetylase histone acetyltransferase |
| Language | English |
| License | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c435t-fa2f643c952e394cec13e947d1090825d9b1bbb5d57cec26e24028e60afbfca23 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 G704-000409.2014.55.5.024 http://ymj.kr/DOIx.php?id=10.3349/ymj.2014.55.5.1333 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.3349/ymj.2014.55.5.1333 |
| PMID | 25048493 |
| PQID | 1547828921 |
| PQPubID | 23479 |
| PageCount | 8 |
| ParticipantIDs | nrf_kci_oai_kci_go_kr_ARTI_330745 pubmedcentral_primary_oai_pubmedcentral_nih_gov_4108820 proquest_miscellaneous_1547828921 pubmed_primary_25048493 crossref_primary_10_3349_ymj_2014_55_5_1333 crossref_citationtrail_10_3349_ymj_2014_55_5_1333 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2014-09-01 |
| PublicationDateYYYYMMDD | 2014-09-01 |
| PublicationDate_xml | – month: 09 year: 2014 text: 2014-09-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | Korea (South) |
| PublicationPlace_xml | – name: Korea (South) |
| PublicationTitle | Yonsei medical journal |
| PublicationTitleAlternate | Yonsei Med J |
| PublicationYear | 2014 |
| Publisher | Yonsei University College of Medicine 연세대학교의과대학 |
| Publisher_xml | – name: Yonsei University College of Medicine – name: 연세대학교의과대학 |
| References | Klose (10.3349/ymj.2014.55.5.1333_ref2) 2006; 31 Akiyama (10.3349/ymj.2014.55.5.1333_ref1) 2006; 103 Sutovsky (10.3349/ymj.2014.55.5.1333_ref21) 2000; 195 Sonnack (10.3349/ymj.2014.55.5.1333_ref12) 2002; 34 Sakkas (10.3349/ymj.2014.55.5.1333_ref28) 2010; 93 Grozinger (10.3349/ymj.2014.55.5.1333_ref29) 2002; 9 de Ruijter (10.3349/ymj.2014.55.5.1333_ref7) 2003; 370 Tesarik (10.3349/ymj.2014.55.5.1333_ref36) 2005; 10 Ma (10.3349/ymj.2014.55.5.1333_ref3) 2008; 319 Gatewood (10.3349/ymj.2014.55.5.1333_ref14) 1990; 265 Hammoud (10.3349/ymj.2014.55.5.1333_ref19) 2009; 460 Emery (10.3349/ymj.2014.55.5.1333_ref17) 2006; 8 Said (10.3349/ymj.2014.55.5.1333_ref34) 2005; 84 Kistler (10.3349/ymj.2014.55.5.1333_ref11) 1996; 225 Practice Committee of the American Society for Reproductive Medicine (10.3349/ymj.2014.55.5.1333_ref31) 2012; 98 Jee (10.3349/ymj.2014.55.5.1333_ref25) 2011; 38 Omisanjo (10.3349/ymj.2014.55.5.1333_ref4) 2007; 127 Fenic (10.3349/ymj.2014.55.5.1333_ref9) 2004; 25 Hecht (10.3349/ymj.2014.55.5.1333_ref22) 2009; 24 Künzle (10.3349/ymj.2014.55.5.1333_ref32) 2003; 79 Spencer (10.3349/ymj.2014.55.5.1333_ref8) 1999; 240 Grunewald (10.3349/ymj.2014.55.5.1333_ref18) 2005; 37 Tesarik (10.3349/ymj.2014.55.5.1333_ref26) 2004; 19 Pasqualotto (10.3349/ymj.2014.55.5.1333_ref33) 2008; 90 Dada (10.3349/ymj.2014.55.5.1333_ref16) 2012; 29 Krawetz (10.3349/ymj.2014.55.5.1333_ref20) 2005; 6 Steger (10.3349/ymj.2014.55.5.1333_ref15) 2011; 34 Steilmann (10.3349/ymj.2014.55.5.1333_ref24) 2011; 23 Robinson (10.3349/ymj.2014.55.5.1333_ref27) 2012; 27 Carrell (10.3349/ymj.2014.55.5.1333_ref35) 2008; 16 Turner (10.3349/ymj.2014.55.5.1333_ref6) 1991; 99 Hazzouri (10.3349/ymj.2014.55.5.1333_ref10) 2000; 79 Faure (10.3349/ymj.2014.55.5.1333_ref13) 2003; 9 Delaval (10.3349/ymj.2014.55.5.1333_ref30) 2007; 26 Rousseaux (10.3349/ymj.2014.55.5.1333_ref23) 2008; 16 Davie (10.3349/ymj.2014.55.5.1333_ref5) 1998; 8 16651529 - Proc Natl Acad Sci U S A. 2006 May 9;103(19):7339-44 16136654 - Nat Rev Genet. 2005 Aug;6(8):633-42 18413057 - Reprod Biomed Online. 2008 Apr;16(4):492-503 19056771 - Hum Reprod. 2009 Mar;24(3):536-45 22791753 - Hum Reprod. 2012 Oct;27(10):2908-17 8660926 - Exp Cell Res. 1996 Jun 15;225(2):374-81 11841934 - Chem Biol. 2002 Jan;9(1):3-16 22959451 - Fertil Steril. 2012 Dec;98(6):1400-6 16491264 - Asian J Androl. 2006 Mar;8(2):131-42 16403636 - Trends Biochem Sci. 2006 Feb;31(2):89-97 16960727 - Histochem Cell Biol. 2007 Feb;127(2):175-81 21128979 - Int J Androl. 2011 Dec;34(6 Pt 1):513-27 14998960 - Hum Reprod. 2004 Mar;19(3):611-5 22384423 - Clin Exp Reprod Med. 2011 Jun;38(2):82-6 17255950 - EMBO J. 2007 Feb 7;26(3):720-9 22127005 - Reprod Fertil Dev. 2011;23(8):997-1011 15820045 - Reprod Biomed Online. 2005 Mar;10(3):370-5 15292114 - J Androl. 2004 Sep-Oct;25(5):811-8 12429021 - Biochem J. 2003 Mar 15;370(Pt 3):737-49 10603574 - Int Rev Cytol. 2000;195:1-65 16026427 - Andrologia. 2005 Jun;37(2-3):69-71 12568836 - Fertil Steril. 2003 Feb;79(2):287-91 14614037 - Mol Hum Reprod. 2003 Dec;9(12):757-63 11152286 - Eur J Cell Biol. 2000 Dec;79(12):950-60 16169398 - Fertil Steril. 2005 Sep;84(3):649-53 10564807 - Gene. 1999 Nov 15;240(1):1-12 12472623 - Andrologia. 2002 Dec;34(6):384-90 9610407 - Curr Opin Genet Dev. 1998 Apr;8(2):173-8 18413055 - Reprod Biomed Online. 2008 Apr;16(4):474-84 2243112 - J Biol Chem. 1990 Nov 25;265(33):20662-6 18462724 - Fertil Steril. 2008 Aug;90(2):278-83 18501342 - Dev Biol. 2008 Jul 1;319(1):110-20 20080235 - Fertil Steril. 2010 Mar 1;93(4):1027-36 1757496 - J Cell Sci. 1991 May;99 ( Pt 1):13-20 19525931 - Nature. 2009 Jul 23;460(7254):473-8 22290605 - J Assist Reprod Genet. 2012 Mar;29(3):213-23 |
| References_xml | – volume: 90 start-page: 278 year: 2008 ident: 10.3349/ymj.2014.55.5.1333_ref33 publication-title: Fertil Steril doi: 10.1016/j.fertnstert.2008.02.123 – volume: 8 start-page: 173 year: 1998 ident: 10.3349/ymj.2014.55.5.1333_ref5 publication-title: Curr Opin Genet Dev doi: 10.1016/S0959-437X(98)80138-X – volume: 24 start-page: 536 year: 2009 ident: 10.3349/ymj.2014.55.5.1333_ref22 publication-title: Hum Reprod doi: 10.1093/humrep/den390 – volume: 79 start-page: 287 year: 2003 ident: 10.3349/ymj.2014.55.5.1333_ref32 publication-title: Fertil Steril doi: 10.1016/S0015-0282(02)04664-2 – volume: 195 start-page: 1 year: 2000 ident: 10.3349/ymj.2014.55.5.1333_ref21 publication-title: Int Rev Cytol doi: 10.1016/S0074-7696(08)62703-5 – volume: 29 start-page: 213 year: 2012 ident: 10.3349/ymj.2014.55.5.1333_ref16 publication-title: J Assist Reprod Genet doi: 10.1007/s10815-012-9715-0 – volume: 27 start-page: 2908 year: 2012 ident: 10.3349/ymj.2014.55.5.1333_ref27 publication-title: Hum Reprod doi: 10.1093/humrep/des261 – volume: 103 start-page: 7339 year: 2006 ident: 10.3349/ymj.2014.55.5.1333_ref1 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0510946103 – volume: 370 start-page: 737 issue: Pt 3 year: 2003 ident: 10.3349/ymj.2014.55.5.1333_ref7 publication-title: Biochem J doi: 10.1042/bj20021321 – volume: 25 start-page: 811 year: 2004 ident: 10.3349/ymj.2014.55.5.1333_ref9 publication-title: J Androl doi: 10.1002/j.1939-4640.2004.tb02859.x – volume: 93 start-page: 1027 year: 2010 ident: 10.3349/ymj.2014.55.5.1333_ref28 publication-title: Fertil Steril doi: 10.1016/j.fertnstert.2009.10.046 – volume: 16 start-page: 474 year: 2008 ident: 10.3349/ymj.2014.55.5.1333_ref35 publication-title: Reprod Biomed Online doi: 10.1016/S1472-6483(10)60454-3 – volume: 265 start-page: 20662 year: 1990 ident: 10.3349/ymj.2014.55.5.1333_ref14 publication-title: J Biol Chem doi: 10.1016/S0021-9258(17)30554-9 – volume: 319 start-page: 110 year: 2008 ident: 10.3349/ymj.2014.55.5.1333_ref3 publication-title: Dev Biol doi: 10.1016/j.ydbio.2008.04.011 – volume: 34 start-page: 384 year: 2002 ident: 10.3349/ymj.2014.55.5.1333_ref12 publication-title: Andrologia doi: 10.1046/j.1439-0272.2002.00524.x – volume: 6 start-page: 633 year: 2005 ident: 10.3349/ymj.2014.55.5.1333_ref20 publication-title: Nat Rev Genet doi: 10.1038/nrg1654 – volume: 31 start-page: 89 year: 2006 ident: 10.3349/ymj.2014.55.5.1333_ref2 publication-title: Trends Biochem Sci doi: 10.1016/j.tibs.2005.12.008 – volume: 26 start-page: 720 year: 2007 ident: 10.3349/ymj.2014.55.5.1333_ref30 publication-title: EMBO J doi: 10.1038/sj.emboj.7601513 – volume: 8 start-page: 131 year: 2006 ident: 10.3349/ymj.2014.55.5.1333_ref17 publication-title: Asian J Androl doi: 10.1111/j.1745-7262.2006.00127.x – volume: 10 start-page: 370 year: 2005 ident: 10.3349/ymj.2014.55.5.1333_ref36 publication-title: Reprod Biomed Online doi: 10.1016/S1472-6483(10)61798-1 – volume: 240 start-page: 1 year: 1999 ident: 10.3349/ymj.2014.55.5.1333_ref8 publication-title: Gene doi: 10.1016/S0378-1119(99)00405-9 – volume: 84 start-page: 649 year: 2005 ident: 10.3349/ymj.2014.55.5.1333_ref34 publication-title: Fertil Steril doi: 10.1016/j.fertnstert.2005.03.052 – volume: 98 start-page: 1400 year: 2012 ident: 10.3349/ymj.2014.55.5.1333_ref31 publication-title: Fertil Steril doi: 10.1016/j.fertnstert.2012.07.1146 – volume: 37 start-page: 69 year: 2005 ident: 10.3349/ymj.2014.55.5.1333_ref18 publication-title: Andrologia doi: 10.1111/j.1439-0272.2005.00656.x – volume: 23 start-page: 997 year: 2011 ident: 10.3349/ymj.2014.55.5.1333_ref24 publication-title: Reprod Fertil Dev doi: 10.1071/RD10197 – volume: 19 start-page: 611 year: 2004 ident: 10.3349/ymj.2014.55.5.1333_ref26 publication-title: Hum Reprod doi: 10.1093/humrep/deh127 – volume: 79 start-page: 950 year: 2000 ident: 10.3349/ymj.2014.55.5.1333_ref10 publication-title: Eur J Cell Biol doi: 10.1078/0171-9335-00123 – volume: 38 start-page: 82 year: 2011 ident: 10.3349/ymj.2014.55.5.1333_ref25 publication-title: Clin Exp Reprod Med doi: 10.5653/cerm.2011.38.2.82 – volume: 16 start-page: 492 year: 2008 ident: 10.3349/ymj.2014.55.5.1333_ref23 publication-title: Reprod Biomed Online doi: 10.1016/S1472-6483(10)60456-7 – volume: 99 start-page: 13 issue: Pt 1 year: 1991 ident: 10.3349/ymj.2014.55.5.1333_ref6 publication-title: J Cell Sci doi: 10.1242/jcs.99.1.13 – volume: 460 start-page: 473 year: 2009 ident: 10.3349/ymj.2014.55.5.1333_ref19 publication-title: Nature doi: 10.1038/nature08162 – volume: 34 start-page: 513 issue: 6 Pt 1 year: 2011 ident: 10.3349/ymj.2014.55.5.1333_ref15 publication-title: Int J Androl doi: 10.1111/j.1365-2605.2010.01129.x – volume: 9 start-page: 3 year: 2002 ident: 10.3349/ymj.2014.55.5.1333_ref29 publication-title: Chem Biol doi: 10.1016/S1074-5521(02)00092-3 – volume: 127 start-page: 175 year: 2007 ident: 10.3349/ymj.2014.55.5.1333_ref4 publication-title: Histochem Cell Biol doi: 10.1007/s00418-006-0234-x – volume: 225 start-page: 374 year: 1996 ident: 10.3349/ymj.2014.55.5.1333_ref11 publication-title: Exp Cell Res doi: 10.1006/excr.1996.0188 – volume: 9 start-page: 757 year: 2003 ident: 10.3349/ymj.2014.55.5.1333_ref13 publication-title: Mol Hum Reprod doi: 10.1093/molehr/gag101 – reference: 21128979 - Int J Androl. 2011 Dec;34(6 Pt 1):513-27 – reference: 22959451 - Fertil Steril. 2012 Dec;98(6):1400-6 – reference: 2243112 - J Biol Chem. 1990 Nov 25;265(33):20662-6 – reference: 20080235 - Fertil Steril. 2010 Mar 1;93(4):1027-36 – reference: 14998960 - Hum Reprod. 2004 Mar;19(3):611-5 – reference: 10564807 - Gene. 1999 Nov 15;240(1):1-12 – reference: 18413055 - Reprod Biomed Online. 2008 Apr;16(4):474-84 – reference: 16136654 - Nat Rev Genet. 2005 Aug;6(8):633-42 – reference: 18462724 - Fertil Steril. 2008 Aug;90(2):278-83 – reference: 12568836 - Fertil Steril. 2003 Feb;79(2):287-91 – reference: 16169398 - Fertil Steril. 2005 Sep;84(3):649-53 – reference: 16651529 - Proc Natl Acad Sci U S A. 2006 May 9;103(19):7339-44 – reference: 22127005 - Reprod Fertil Dev. 2011;23(8):997-1011 – reference: 22290605 - J Assist Reprod Genet. 2012 Mar;29(3):213-23 – reference: 22791753 - Hum Reprod. 2012 Oct;27(10):2908-17 – reference: 8660926 - Exp Cell Res. 1996 Jun 15;225(2):374-81 – reference: 16491264 - Asian J Androl. 2006 Mar;8(2):131-42 – reference: 16960727 - Histochem Cell Biol. 2007 Feb;127(2):175-81 – reference: 11152286 - Eur J Cell Biol. 2000 Dec;79(12):950-60 – reference: 12429021 - Biochem J. 2003 Mar 15;370(Pt 3):737-49 – reference: 16026427 - Andrologia. 2005 Jun;37(2-3):69-71 – reference: 19056771 - Hum Reprod. 2009 Mar;24(3):536-45 – reference: 18501342 - Dev Biol. 2008 Jul 1;319(1):110-20 – reference: 9610407 - Curr Opin Genet Dev. 1998 Apr;8(2):173-8 – reference: 11841934 - Chem Biol. 2002 Jan;9(1):3-16 – reference: 10603574 - Int Rev Cytol. 2000;195:1-65 – reference: 16403636 - Trends Biochem Sci. 2006 Feb;31(2):89-97 – reference: 22384423 - Clin Exp Reprod Med. 2011 Jun;38(2):82-6 – reference: 1757496 - J Cell Sci. 1991 May;99 ( Pt 1):13-20 – reference: 15820045 - Reprod Biomed Online. 2005 Mar;10(3):370-5 – reference: 17255950 - EMBO J. 2007 Feb 7;26(3):720-9 – reference: 12472623 - Andrologia. 2002 Dec;34(6):384-90 – reference: 14614037 - Mol Hum Reprod. 2003 Dec;9(12):757-63 – reference: 15292114 - J Androl. 2004 Sep-Oct;25(5):811-8 – reference: 19525931 - Nature. 2009 Jul 23;460(7254):473-8 – reference: 18413057 - Reprod Biomed Online. 2008 Apr;16(4):492-503 |
| SSID | ssj0027102 |
| Score | 2.1775157 |
| Snippet | The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated... Purpose: The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in... |
| SourceID | nrf pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 1333 |
| SubjectTerms | Acetylation Adult DNA Fragmentation Epigenesis, Genetic Histone Acetyltransferases - metabolism Histones - metabolism Humans Immunohistochemistry Male Middle Aged Original Protein Processing, Post-Translational Semen Analysis Spermatozoa - metabolism 의학일반 |
| Title | Histone Acetylation Level and Histone Acetyltransferase/Deacetylase Activity in Ejaculated Sperm from Normozoospermic Men |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25048493 https://www.proquest.com/docview/1547828921 https://pubmed.ncbi.nlm.nih.gov/PMC4108820 https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001903947 |
| Volume | 55 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Yonsei Medical Journal, 2014, 55(5), , pp.1333-1340 |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1bb9MwFLbWISFeEHfGpTISbyhdnNhJ84DQBJsGonuBir1ZsWOP7uKONJMov57vJGmhaCCRl0ixHTs-ts_3xcfnMPbSidyK2JvIxeMyklniIyg9TDxP4Nh46FQ6jTw5yg6n8sOxOt5iq3BHfQcurqV2FE9qWp-Pvn9bvsGEf02MM5XF7vLilGy05EipkRqBdKUDdgOaKSMyNpHjXwRMtDaIGIdpRGcwu0M0f3nHhqIahNpfh0H_NKX8TTcd3GG3e1DJ97pRcJdtuXCP3Zz02-b32bJ1BhIc37OuWXbWb_wjmQvxMlR8M7Vpoayrod5232HBbEssKLULNMFnge-flmS_CrTKP11ibed0SoUfAf_Of8xb5-MXM8snLjxg04P9z28Poz7mQmQBnJrIl4kHSLGFSlxaSOusSF0h84oMOMEmq8IIY4yqVI60JHO0OzN2WVx6422ZpA_ZdkCTHzNuZY7HOShQGctKysLiyn2WZqnBMBA7TKw6WNveITnFxTjXICYkFA2haBKKVkorTULZYa_WZS47dxz_zP0CctNndqbJizbdT-b6rNbgCu9RCPBJIc9Kqhozi7ZLyuDmVwstyNUZ-GiClj7qpLyukxy_jWWBGvIN-a8zUH2bKWH2tfXeLQWxmvjJf33JU3aLHnTGbc_YdlNfuedAQ40ZsoGKvgzbfwnDdrj_BKL3CfY |
| linkProvider | Scholars Portal |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Histone+Acetylation+Level+and+Histone+Acetyltransferase%2FDeacetylase+Activity+in+Ejaculated+Sperm+from+Normozoospermic+Men&rft.jtitle=Yonsei+medical+journal&rft.au=Kim%2C+Jee+Hyun&rft.au=Jee%2C+Byung+Chul&rft.au=Lee%2C+Jang+Mi&rft.au=Suh%2C+Chang+Suk&rft.date=2014-09-01&rft.issn=0513-5796&rft.eissn=1976-2437&rft.volume=55&rft.issue=5&rft.spage=1333&rft_id=info:doi/10.3349%2Fymj.2014.55.5.1333&rft.externalDBID=n%2Fa&rft.externalDocID=10_3349_ymj_2014_55_5_1333 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0513-5796&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0513-5796&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0513-5796&client=summon |