A Diverse Lipid Antigen–Specific TCR Repertoire Is Clonally Expanded during Active Tuberculosis

• Published in: The Journal of immunology (1950) Vol. 201; no. 3; pp. 888 - 896
• Main Authors: DeWitt, William S, Yu, Krystle K Q, Wilburn, Damien B, Sherwood, Anna, Vignali, Marissa, Day, Cheryl L, Scriba, Thomas J, Robins, Harlan S, Swanson, Willie J, Emerson, Ryan O, Bradley, Philip H, Seshadri, Chetan
• Format: Journal Article
• Language: English
• Published: England American Association of Immunologists 01.08.2018
• Subjects: Adolescent; Antigens, CD1 - immunology; Cell Line, Tumor; Cells, Cultured; Child; Female; Humans; K562 Cells; Lipids; Lipids - immunology; Lymphocytes; Lymphocytes T; Major histocompatibility complex; Male; Mycobacterium - immunology; Receptors, Antigen, T-Cell - immunology; T cell receptors; T-Lymphocytes; Tuberculosis; Tuberculosis - immunology

Human T cells that recognize lipid Ags presented by highly conserved CD1 proteins often express semi-invariant TCRs, but the true diversity of lipid Ag–specific TCRs remains unknown. We use CD1b tetramers and high-throughput immunosequencing to analyze thousands of TCRs from ex vivo–sorted or in vit...