Methylation of TFAM gene promoter in peripheral white blood cells is associated with insulin resistance in adolescents
To explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of adolescents with features of metabolic syndrome. The data and blood samples were collected from 122 adolescents out of a cross-sectional study of 934 hi...
Saved in:
Published in | Molecular genetics and metabolism Vol. 100; no. 1; pp. 83 - 87 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.05.2010
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | To explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of adolescents with features of metabolic syndrome.
The data and blood samples were collected from 122 adolescents out of a cross-sectional study of 934 high-school students. The population was divided into two groups: noninsulin resistance (NIR) and insulin resistance (IR). After bisulfite treatment of genomic DNA from peripheral leukocytes, we used methylation-specific polymerase chain reaction (PCR) to assess DNA methylation of three putative methylation target sites (CpG) in the TFAM promoter.
The ratio of the promoter methylated DNA/unmethylated DNA was 0.012±0.0009 (1.2% of alleles), and inversely correlated with the biochemical features of insulin resistance (plasma fasting insulin R: −0.26, p<0.004 and homeostasis model assessment (HOMA) index R: −0.27, p<0.002), and obesity (R: −0.27, p<0.002). Multiple regression analysis showed that the log-transformed HOMA index correlated with the status of promoter methylation of TFAM, independently of body mass index (BMI) Z score (β: −0.33±0.094, p=0.00094). Finally, the TFAM promoter methylated DNA/unmethylated DNA ratio was found to be significantly associated with insulin resistance as dichotomous variable (NIR n=45, 0.014±0.002 and IR n=77, 0.011±0.001, respectively, p<0.016).
Our findings suggest a potential role of promoter TFAM methylation in the pathogenesis of insulin resistance in adolescents. |
---|---|
AbstractList | PURPOSETo explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of adolescents with features of metabolic syndrome.METHODSThe data and blood samples were collected from 122 adolescents out of a cross-sectional study of 934 high-school students. The population was divided into two groups: noninsulin resistance (NIR) and insulin resistance (IR). After bisulfite treatment of genomic DNA from peripheral leukocytes, we used methylation-specific polymerase chain reaction (PCR) to assess DNA methylation of three putative methylation target sites (CpG) in the TFAM promoter.RESULTSThe ratio of the promoter methylated DNA/unmethylated DNA was 0.012+/-0.0009 (1.2% of alleles), and inversely correlated with the biochemical features of insulin resistance (plasma fasting insulin R: -0.26, p<0.004 and homeostasis model assessment (HOMA) index R: -0.27, p<0.002), and obesity (R: -0.27, p<0.002). Multiple regression analysis showed that the log-transformed HOMA index correlated with the status of promoter methylation of TFAM, independently of body mass index (BMI) Z score (beta: -0.33+/-0.094, p=0.00094). Finally, the TFAM promoter methylated DNA/unmethylated DNA ratio was found to be significantly associated with insulin resistance as dichotomous variable (NIR n=45, 0.014+/-0.002 and IR n=77, 0.011+/-0.001, respectively, p<0.016).CONCLUSIONOur findings suggest a potential role of promoter TFAM methylation in the pathogenesis of insulin resistance in adolescents. To explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of adolescents with features of metabolic syndrome. The data and blood samples were collected from 122 adolescents out of a cross-sectional study of 934 high-school students. The population was divided into two groups: noninsulin resistance (NIR) and insulin resistance (IR). After bisulfite treatment of genomic DNA from peripheral leukocytes, we used methylation-specific polymerase chain reaction (PCR) to assess DNA methylation of three putative methylation target sites (CpG) in the TFAM promoter. The ratio of the promoter methylated DNA/unmethylated DNA was 0.012+/-0.0009 (1.2% of alleles), and inversely correlated with the biochemical features of insulin resistance (plasma fasting insulin R: -0.26, p<0.004 and homeostasis model assessment (HOMA) index R: -0.27, p<0.002), and obesity (R: -0.27, p<0.002). Multiple regression analysis showed that the log-transformed HOMA index correlated with the status of promoter methylation of TFAM, independently of body mass index (BMI) Z score (beta: -0.33+/-0.094, p=0.00094). Finally, the TFAM promoter methylated DNA/unmethylated DNA ratio was found to be significantly associated with insulin resistance as dichotomous variable (NIR n=45, 0.014+/-0.002 and IR n=77, 0.011+/-0.001, respectively, p<0.016). Our findings suggest a potential role of promoter TFAM methylation in the pathogenesis of insulin resistance in adolescents. Purpose - To explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of adolescents with features of metabolic syndrome. Methods - The data and blood samples were collected from 122 adolescents out of a cross-sectional study of 934 high-school students. The population was divided into two groups: noninsulin resistance (NIR) and insulin resistance (IR). After bisulfite treatment of genomic DNA from peripheral leukocytes, we used methylation-specific polymerase chain reaction (PCR) to assess DNA methylation of three putative methylation target sites (CpG) in the TFAM promoter. Results - The ratio of the promoter methylated DNA/unmethylated DNA was 0.012 +/- 0.0009 (1.2% of alleles), and inversely correlated with the biochemical features of insulin resistance (plasma fasting insulin R: -0.26, p < 0.004 and homeostasis model assessment (HOMA) index R: -0.27, p < 0.002), and obesity (R: -0.27, p < 0.002). Multiple regression analysis showed that the log-transformed HOMA index correlated with the status of promoter methylation of TFAM, independently of body mass index (BMI) Z score (b: -0.33 +/- 0.094, p = 0.00094). Finally, the TFAM promoter methylated DNA/unmethylated DNA ratio was found to be significantly associated with insulin resistance as dichotomous variable (NIR n = 45, 0.014 +/- 0.002 and IR n = 77, 0.011 +/- 0.001, respectively, p < 0.016). Conclusion - Our findings suggest a potential role of promoter TFAM methylation in the pathogenesis of insulin resistance in adolescents. To explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of adolescents with features of metabolic syndrome. The data and blood samples were collected from 122 adolescents out of a cross-sectional study of 934 high-school students. The population was divided into two groups: noninsulin resistance (NIR) and insulin resistance (IR). After bisulfite treatment of genomic DNA from peripheral leukocytes, we used methylation-specific polymerase chain reaction (PCR) to assess DNA methylation of three putative methylation target sites (CpG) in the TFAM promoter. The ratio of the promoter methylated DNA/unmethylated DNA was 0.012±0.0009 (1.2% of alleles), and inversely correlated with the biochemical features of insulin resistance (plasma fasting insulin R: −0.26, p<0.004 and homeostasis model assessment (HOMA) index R: −0.27, p<0.002), and obesity (R: −0.27, p<0.002). Multiple regression analysis showed that the log-transformed HOMA index correlated with the status of promoter methylation of TFAM, independently of body mass index (BMI) Z score (β: −0.33±0.094, p=0.00094). Finally, the TFAM promoter methylated DNA/unmethylated DNA ratio was found to be significantly associated with insulin resistance as dichotomous variable (NIR n=45, 0.014±0.002 and IR n=77, 0.011±0.001, respectively, p<0.016). Our findings suggest a potential role of promoter TFAM methylation in the pathogenesis of insulin resistance in adolescents. |
Author | Dieuzeide, Guillermo Pirola, Carlos J. Sookoian, Silvia González, Claudio D. García, Silvia I. Gemma, Carolina Gianotti, Tomas Fernández |
Author_xml | – sequence: 1 givenname: Carolina surname: Gemma fullname: Gemma, Carolina organization: Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research, A. Lanari-IDIM, University of Buenos Aires, National Council of Scientific and Technological Research (CONICET), Combatientes de Malvinas 3150, Ciudad Autónoma de Buenos Aires C1427ARO, Argentina – sequence: 2 givenname: Silvia surname: Sookoian fullname: Sookoian, Silvia organization: Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research, A. Lanari-IDIM, University of Buenos Aires, National Council of Scientific and Technological Research (CONICET), Combatientes de Malvinas 3150, Ciudad Autónoma de Buenos Aires C1427ARO, Argentina – sequence: 3 givenname: Guillermo surname: Dieuzeide fullname: Dieuzeide, Guillermo organization: CAIDEM, Chacabuco, Argentina – sequence: 4 givenname: Silvia I. surname: García fullname: García, Silvia I. organization: Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research, A. Lanari-IDIM, University of Buenos Aires, National Council of Scientific and Technological Research (CONICET), Combatientes de Malvinas 3150, Ciudad Autónoma de Buenos Aires C1427ARO, Argentina – sequence: 5 givenname: Tomas Fernández surname: Gianotti fullname: Gianotti, Tomas Fernández organization: Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research, A. Lanari-IDIM, University of Buenos Aires, National Council of Scientific and Technological Research (CONICET), Combatientes de Malvinas 3150, Ciudad Autónoma de Buenos Aires C1427ARO, Argentina – sequence: 6 givenname: Claudio D. surname: González fullname: González, Claudio D. organization: Department of Pharmacology, School of Medicine, University of Buenos Aires, Argentina – sequence: 7 givenname: Carlos J. surname: Pirola fullname: Pirola, Carlos J. email: pirola.carlos@lanari.fmed.uba.ar, cpirola@ciudad.com.ar organization: Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research, A. Lanari-IDIM, University of Buenos Aires, National Council of Scientific and Technological Research (CONICET), Combatientes de Malvinas 3150, Ciudad Autónoma de Buenos Aires C1427ARO, Argentina |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20202876$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkUtPJCEUhclEM77mF0xi2M2qW14NVYtZGOMr0bjRNaHgtk2nqujh0pr-99K2uhwDCYR851zuPUdkb0wjEPKbsylnXJ8tp5vheYCpYPWFiSlj6gc55KzVEyOY3vu881YckCPEJWOcz1r1kxwIVldj9CF5uYey2PSuxDTSNKePV-f39BlGoKuchlQg0zjSFeS4WkB2PX1dxAK061MK1EPfI41IHWLy0RUI9DWWRZXguq-6DBixuNHD1sWF1AN6GAuekP256xF-fZzH5Onq8vHiZnL3cH17cX438UrOygT0rJPAwPPakeBeG9eAca0JULdsO_CNgc5xL7iWgQeuuYY2aMObplNzeUz-7HxrN__WgMUOEbffdiOkNVqjlG5la8T3pJRazVSjKil3pM8JMcPcrnIcXN5Yzuw2Gbu078nYbTKWCVuTqarTD_91N0D40nxGUYG_OwDqPF4iZIs-Qh1diBl8sSHF_xZ4Aysyo94 |
CitedBy_id | crossref_primary_10_1002_term_2775 crossref_primary_10_1096_fj_201901072R crossref_primary_10_3390_biomedicines10123065 crossref_primary_10_1007_s12263_015_0471_5 crossref_primary_10_2217_epi_15_7 crossref_primary_10_4161_cc_28189 crossref_primary_10_1016_j_jacl_2012_04_079 crossref_primary_10_1038_pr_2013_2 crossref_primary_10_1007_s11010_024_05001_4 crossref_primary_10_1007_s11906_010_0164_9 crossref_primary_10_1016_j_jnutbio_2013_05_002 crossref_primary_10_1002_hep_23927 crossref_primary_10_2174_1871530319666190301145545 crossref_primary_10_1016_j_mam_2012_06_010 crossref_primary_10_1038_s41598_019_38980_2 crossref_primary_10_3390_ijms23010295 crossref_primary_10_1007_s13105_017_0560_6 crossref_primary_10_1089_ars_2014_6186 crossref_primary_10_1080_17474124_2020_1765772 crossref_primary_10_1371_journal_pone_0031821 crossref_primary_10_2337_db11_1048 crossref_primary_10_1016_j_iac_2014_07_004 crossref_primary_10_14348_molcells_2015_0005 crossref_primary_10_1016_j_metabol_2014_05_014 crossref_primary_10_1371_journal_pone_0113784 crossref_primary_10_1007_s11892_012_0361_9 crossref_primary_10_1016_j_chemosphere_2020_127305 crossref_primary_10_1038_hr_2011_133 crossref_primary_10_17795_gct_21227 crossref_primary_10_1016_j_soard_2013_12_019 crossref_primary_10_1194_jlr_M048504 crossref_primary_10_3390_nu12061889 crossref_primary_10_1016_j_bbrc_2024_149742 crossref_primary_10_1042_BCJ20160008 crossref_primary_10_17795_gct_18308 crossref_primary_10_1262_jrd_2016_168 crossref_primary_10_1007_s13105_014_0341_4 crossref_primary_10_1007_s40142_019_00176_5 crossref_primary_10_1186_s13148_023_01556_z |
Cites_doi | 10.1038/oby.2008.253 10.1172/JCI30938 10.1016/j.jchromb.2005.11.031 10.1038/ng0398-231 10.2337/diabetes.48.4.685 10.1101/gad.13.1.26 10.1291/hypres.30.5 10.1016/j.amjhyper.2005.04.014 10.1677/jme.0.0290265 10.1203/01.PDR.0000119367.21770.D7 10.1007/s00125-007-0916-5 10.2337/dc06-0709 10.1093/bioinformatics/18.11.1427 10.1016/S0304-3940(02)01423-4 10.1006/meth.2001.1262 10.1016/j.ceb.2007.04.011 10.1161/CIRCRESAHA.107.165472 10.1007/s11154-007-9050-4 10.1056/NEJMoa031314 10.1038/oby.2008.605 10.1038/ng1990 10.1016/S0168-8227(01)00330-8 10.1038/nrg2341 |
ContentType | Journal Article |
Copyright | 2010 Elsevier Inc. (c) 2010 Elsevier Inc. All rights reserved. |
Copyright_xml | – notice: 2010 Elsevier Inc. – notice: (c) 2010 Elsevier Inc. All rights reserved. |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 7T5 7TM 8FD FR3 H94 P64 RC3 |
DOI | 10.1016/j.ymgme.2010.02.004 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic Immunology Abstracts Nucleic Acids Abstracts Technology Research Database Engineering Research Database AIDS and Cancer Research Abstracts Biotechnology and BioEngineering Abstracts Genetics Abstracts |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic Genetics Abstracts Technology Research Database Nucleic Acids Abstracts AIDS and Cancer Research Abstracts Immunology Abstracts Engineering Research Database Biotechnology and BioEngineering Abstracts |
DatabaseTitleList | MEDLINE - Academic MEDLINE Genetics Abstracts |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology Chemistry Biology |
EISSN | 1096-7206 |
EndPage | 87 |
ExternalDocumentID | 10_1016_j_ymgme_2010_02_004 20202876 S1096719210000521 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K --M -~X .55 .GJ .~1 0R~ 123 1B1 1RT 1~. 1~5 29M 4.4 457 4G. 53G 5RE 5VS 7-5 71M 8P~ AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACRLP ADBBV ADEZE ADFGL ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CAG COF CS3 DM4 DOVZS DU5 EBS EFBJH EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-Q GBLVA HVGLF HZ~ IHE J1W K-O KOM L7B LG5 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SDF SDG SDP SES SEW SPCBC SSU SSZ T5K X7M XPP ZA5 ZGI ZMT ZU3 ~G- ~KM AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 7T5 7TM 8FD FR3 H94 P64 RC3 |
ID | FETCH-LOGICAL-c435t-e65b3e0ec172021c67a8e7a97de7de39bec87eba1c2163d1d1616e9d67188b4f3 |
IEDL.DBID | .~1 |
ISSN | 1096-7192 |
IngestDate | Sat Aug 17 00:24:42 EDT 2024 Sat Aug 17 03:27:13 EDT 2024 Thu Sep 26 17:22:00 EDT 2024 Sat Sep 28 07:47:03 EDT 2024 Fri Feb 23 02:21:45 EST 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | DNA methylation Epigenetics Insulin resistance TFAM Adolescents Metabolic syndrome |
Language | English |
License | (c) 2010 Elsevier Inc. All rights reserved. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c435t-e65b3e0ec172021c67a8e7a97de7de39bec87eba1c2163d1d1616e9d67188b4f3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
OpenAccessLink | https://ri.conicet.gov.ar/bitstream/11336/103132/5/CONICET_Digital_Nro.d364da4d-d5fe-43ad-9f2f-e514088bdbc3_D.pdf |
PMID | 20202876 |
PQID | 733645484 |
PQPubID | 23479 |
PageCount | 5 |
ParticipantIDs | proquest_miscellaneous_744693972 proquest_miscellaneous_733645484 crossref_primary_10_1016_j_ymgme_2010_02_004 pubmed_primary_20202876 elsevier_sciencedirect_doi_10_1016_j_ymgme_2010_02_004 |
PublicationCentury | 2000 |
PublicationDate | May 2010 2010-May 2010-05-00 20100501 |
PublicationDateYYYYMMDD | 2010-05-01 |
PublicationDate_xml | – month: 05 year: 2010 text: May 2010 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Molecular genetics and metabolism |
PublicationTitleAlternate | Mol Genet Metab |
PublicationYear | 2010 |
Publisher | Elsevier Inc |
Publisher_xml | – name: Elsevier Inc |
References | Ritov, Menshikova, Kelley (bib6) 2006; 831 Walsh, Bestor (bib24) 1999; 13 Lee, Lee, Im, Shim, Kim, Lee (bib12) 2007; 30 Ling, Poulsen, Simonsson, Ronn, Holmkvist, Almgren, Hagert, Nilsson, Mabey, Nilsson, Vaag, Groop (bib7) 2007; 117 Choi, Kim, Pak (bib26) 2001; 54 Gianotti, Sookoian, Dieuzeide, Garcia, Gemma, Gonzalez, Pirola (bib9) 2008; 16 Yokomori, Tawata, Onaya (bib20) 1999; 48 Devaskar, Thamotharan (bib2) 2007; 8 Ling, Del, Lupi, Ronn, Granhall, Luthman, Masiello, Marchetti, Groop, Del (bib21) 2008; 51 Park, Stoffers, Nicholls, Simmons (bib3) 2008; 118 Livak, Schmittgen (bib16) 2001; 25 Grabe (bib18) 2002; 2 Kawasaki (bib13) 1990 Samson, Wong (bib22) 2002; 29 Gemma, Sookoian, Alvarinas, Garcia, Quintana, Kanevsky, Gonzalez, Pirola (bib14) 2009; 17 Li, Dahiya (bib15) 2002; 18 Petersen, Dufour, Befroy, Garcia, Shulman (bib5) 2004; 350 Larsson, Wang, Wilhelmsson, Oldfors, Rustin, Lewandoski, Barsh, Clayton (bib8) 1998; 18 Lee, Okumura, Davis, Herman, Gurney (bib1) 2006; 29 Sookoian, Garcia, Gianotti, Dieuzeide, Gonzalez, Pirola (bib11) 2005; 18 Suzuki, Bird (bib23) 2008; 9 Weber, Hellmann, Stadler, Ramos, Paabo, Rebhan, Schubeler (bib25) 2007; 39 Porto, Garcia, Dieuzeide, Gonzalez, Landa, Pirola (bib10) 2004; 55 Kim, Wei, Sowers (bib4) 2008; 102 Weber, Schubeler (bib19) 2007; 19 Ramakers, Ruijter, Deprez, Moorman (bib17) 2003; 339 Suzuki (10.1016/j.ymgme.2010.02.004_bib23) 2008; 9 Livak (10.1016/j.ymgme.2010.02.004_bib16) 2001; 25 Grabe (10.1016/j.ymgme.2010.02.004_bib18) 2002; 2 Ling (10.1016/j.ymgme.2010.02.004_bib21) 2008; 51 Samson (10.1016/j.ymgme.2010.02.004_bib22) 2002; 29 Kawasaki (10.1016/j.ymgme.2010.02.004_bib13) 1990 Porto (10.1016/j.ymgme.2010.02.004_bib10) 2004; 55 Yokomori (10.1016/j.ymgme.2010.02.004_bib20) 1999; 48 Devaskar (10.1016/j.ymgme.2010.02.004_bib2) 2007; 8 Petersen (10.1016/j.ymgme.2010.02.004_bib5) 2004; 350 Ramakers (10.1016/j.ymgme.2010.02.004_bib17) 2003; 339 Sookoian (10.1016/j.ymgme.2010.02.004_bib11) 2005; 18 Choi (10.1016/j.ymgme.2010.02.004_bib26) 2001; 54 Kim (10.1016/j.ymgme.2010.02.004_bib4) 2008; 102 Gianotti (10.1016/j.ymgme.2010.02.004_bib9) 2008; 16 Ling (10.1016/j.ymgme.2010.02.004_bib7) 2007; 117 Lee (10.1016/j.ymgme.2010.02.004_bib1) 2006; 29 Lee (10.1016/j.ymgme.2010.02.004_bib12) 2007; 30 Walsh (10.1016/j.ymgme.2010.02.004_bib24) 1999; 13 Larsson (10.1016/j.ymgme.2010.02.004_bib8) 1998; 18 Ritov (10.1016/j.ymgme.2010.02.004_bib6) 2006; 831 Li (10.1016/j.ymgme.2010.02.004_bib15) 2002; 18 Gemma (10.1016/j.ymgme.2010.02.004_bib14) 2009; 17 Weber (10.1016/j.ymgme.2010.02.004_bib25) 2007; 39 Weber (10.1016/j.ymgme.2010.02.004_bib19) 2007; 19 Park (10.1016/j.ymgme.2010.02.004_bib3) 2008; 118 |
References_xml | – volume: 51 start-page: 615 year: 2008 end-page: 622 ident: bib21 article-title: Epigenetic regulation of PPARGC1A in human type 2 diabetic islets and effect on insulin secretion publication-title: Diabetologia contributor: fullname: Del – volume: 54 start-page: S3 year: 2001 end-page: S9 ident: bib26 article-title: Mitochondrial transcription factor A (mtTFA) and diabetes publication-title: Diabetes Res. Clin. Pract. contributor: fullname: Pak – volume: 16 start-page: 1591 year: 2008 end-page: 1595 ident: bib9 article-title: A decreased mitochondrial DNA content is related to insulin resistance in adolescents publication-title: Obesity contributor: fullname: Pirola – volume: 339 start-page: 62 year: 2003 end-page: 66 ident: bib17 article-title: Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data publication-title: Neurosci. Lett. contributor: fullname: Moorman – volume: 39 start-page: 457 year: 2007 end-page: 466 ident: bib25 article-title: Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome publication-title: Nat. Genet. contributor: fullname: Schubeler – volume: 118 start-page: 2316 year: 2008 end-page: 2324 ident: bib3 article-title: Development of type 2 diabetes following intrauterine growth retardation in rats is associated with progressive epigenetic silencing of Pdx1 publication-title: J. Clin. Invest. contributor: fullname: Simmons – volume: 18 start-page: 1427 year: 2002 end-page: 1431 ident: bib15 article-title: MethPrimer: designing primers for methylation PCRs publication-title: Bioinformatics contributor: fullname: Dahiya – volume: 102 start-page: 401 year: 2008 end-page: 414 ident: bib4 article-title: Role of mitochondrial dysfunction in insulin resistance publication-title: Circ. Res. contributor: fullname: Sowers – volume: 17 start-page: 1032 year: 2009 end-page: 1039 ident: bib14 article-title: Maternal pregestational BMI is associated with methylation of the PPARGC1A promoter in newborns publication-title: Obesity contributor: fullname: Pirola – volume: 19 start-page: 273 year: 2007 end-page: 280 ident: bib19 article-title: Genomic patterns of DNA methylation: targets and function of an epigenetic mark publication-title: Curr. Opin. Cell Biol. contributor: fullname: Schubeler – volume: 30 start-page: 5 year: 2007 end-page: 11 ident: bib12 article-title: Insulin resistance is associated with arterial stiffness independent of obesity in male adolescents publication-title: Hypertens. Res. contributor: fullname: Lee – volume: 9 start-page: 465 year: 2008 end-page: 476 ident: bib23 article-title: DNA methylation landscapes: provocative insights from epigenomics publication-title: Nat. Rev. Genet. contributor: fullname: Bird – volume: 8 start-page: 105 year: 2007 end-page: 113 ident: bib2 article-title: Metabolic programming in the pathogenesis of insulin resistance publication-title: Rev. Endocr. Metab. Disord. contributor: fullname: Thamotharan – volume: 2 start-page: S1 year: 2002 end-page: S15 ident: bib18 article-title: AliBaba2: context specific identification of transcription factor binding sites publication-title: In Silico. Biol. contributor: fullname: Grabe – volume: 18 start-page: 1271 year: 2005 end-page: 1275 ident: bib11 article-title: The G-308A promoter variant of the tumor necrosis factor-alpha gene is associated with hypertension in adolescents harboring the metabolic syndrome publication-title: Am. J. Hypertens. contributor: fullname: Pirola – volume: 18 start-page: 231 year: 1998 end-page: 236 ident: bib8 article-title: Mitochondrial transcription factor A is necessary for mtDNA maintenance and embryogenesis in mice publication-title: Nat. Genet. contributor: fullname: Clayton – volume: 25 start-page: 402 year: 2001 end-page: 408 ident: bib16 article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method publication-title: Methods contributor: fullname: Schmittgen – volume: 350 start-page: 664 year: 2004 end-page: 671 ident: bib5 article-title: Impaired mitochondrial activity in the insulin-resistant offspring of patients with type 2 diabetes publication-title: N. Engl. J. Med. contributor: fullname: Shulman – volume: 29 start-page: 2427 year: 2006 end-page: 2432 ident: bib1 article-title: Prevalence and determinants of insulin resistance among US adolescents: a population-based study publication-title: Diabetes Care contributor: fullname: Gurney – start-page: 146 year: 1990 end-page: 152 ident: bib13 article-title: Sample preparation from blood, cells, and other fluids publication-title: PCR Protocols. A guide to Methods and Applications contributor: fullname: Kawasaki – volume: 831 start-page: 63 year: 2006 end-page: 71 ident: bib6 article-title: Analysis of cardiolipin in human muscle biopsy publication-title: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. contributor: fullname: Kelley – volume: 13 start-page: 26 year: 1999 end-page: 34 ident: bib24 article-title: Cytosine methylation and mammalian development publication-title: Genes Dev. contributor: fullname: Bestor – volume: 55 start-page: 836 year: 2004 end-page: 841 ident: bib10 article-title: Clinical features of the metabolic syndrome in adolescents: minor role of the Trp64Arg beta3-adrenergic receptor gene variant publication-title: Pediatr. Res. contributor: fullname: Pirola – volume: 117 start-page: 3427 year: 2007 end-page: 3435 ident: bib7 article-title: Genetic and epigenetic factors are associated with expression of respiratory chain component NDUFB6 in human skeletal muscle publication-title: J. Clin. Invest. contributor: fullname: Groop – volume: 48 start-page: 685 year: 1999 end-page: 690 ident: bib20 article-title: DNA demethylation during the differentiation of 3T3-L1 cells affects the expression of the mouse GLUT4 gene publication-title: Diabetes contributor: fullname: Onaya – volume: 29 start-page: 265 year: 2002 end-page: 279 ident: bib22 article-title: Role of Sp1 in insulin regulation of gene expression publication-title: J. Mol. Endocrinol. contributor: fullname: Wong – volume: 16 start-page: 1591 year: 2008 ident: 10.1016/j.ymgme.2010.02.004_bib9 article-title: A decreased mitochondrial DNA content is related to insulin resistance in adolescents publication-title: Obesity doi: 10.1038/oby.2008.253 contributor: fullname: Gianotti – volume: 117 start-page: 3427 year: 2007 ident: 10.1016/j.ymgme.2010.02.004_bib7 article-title: Genetic and epigenetic factors are associated with expression of respiratory chain component NDUFB6 in human skeletal muscle publication-title: J. Clin. Invest. doi: 10.1172/JCI30938 contributor: fullname: Ling – volume: 831 start-page: 63 year: 2006 ident: 10.1016/j.ymgme.2010.02.004_bib6 article-title: Analysis of cardiolipin in human muscle biopsy publication-title: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. doi: 10.1016/j.jchromb.2005.11.031 contributor: fullname: Ritov – volume: 18 start-page: 231 year: 1998 ident: 10.1016/j.ymgme.2010.02.004_bib8 article-title: Mitochondrial transcription factor A is necessary for mtDNA maintenance and embryogenesis in mice publication-title: Nat. Genet. doi: 10.1038/ng0398-231 contributor: fullname: Larsson – volume: 48 start-page: 685 year: 1999 ident: 10.1016/j.ymgme.2010.02.004_bib20 article-title: DNA demethylation during the differentiation of 3T3-L1 cells affects the expression of the mouse GLUT4 gene publication-title: Diabetes doi: 10.2337/diabetes.48.4.685 contributor: fullname: Yokomori – volume: 13 start-page: 26 year: 1999 ident: 10.1016/j.ymgme.2010.02.004_bib24 article-title: Cytosine methylation and mammalian development publication-title: Genes Dev. doi: 10.1101/gad.13.1.26 contributor: fullname: Walsh – volume: 30 start-page: 5 year: 2007 ident: 10.1016/j.ymgme.2010.02.004_bib12 article-title: Insulin resistance is associated with arterial stiffness independent of obesity in male adolescents publication-title: Hypertens. Res. doi: 10.1291/hypres.30.5 contributor: fullname: Lee – volume: 118 start-page: 2316 year: 2008 ident: 10.1016/j.ymgme.2010.02.004_bib3 article-title: Development of type 2 diabetes following intrauterine growth retardation in rats is associated with progressive epigenetic silencing of Pdx1 publication-title: J. Clin. Invest. contributor: fullname: Park – volume: 18 start-page: 1271 year: 2005 ident: 10.1016/j.ymgme.2010.02.004_bib11 article-title: The G-308A promoter variant of the tumor necrosis factor-alpha gene is associated with hypertension in adolescents harboring the metabolic syndrome publication-title: Am. J. Hypertens. doi: 10.1016/j.amjhyper.2005.04.014 contributor: fullname: Sookoian – volume: 29 start-page: 265 year: 2002 ident: 10.1016/j.ymgme.2010.02.004_bib22 article-title: Role of Sp1 in insulin regulation of gene expression publication-title: J. Mol. Endocrinol. doi: 10.1677/jme.0.0290265 contributor: fullname: Samson – volume: 55 start-page: 836 year: 2004 ident: 10.1016/j.ymgme.2010.02.004_bib10 article-title: Clinical features of the metabolic syndrome in adolescents: minor role of the Trp64Arg beta3-adrenergic receptor gene variant publication-title: Pediatr. Res. doi: 10.1203/01.PDR.0000119367.21770.D7 contributor: fullname: Porto – volume: 51 start-page: 615 year: 2008 ident: 10.1016/j.ymgme.2010.02.004_bib21 article-title: Epigenetic regulation of PPARGC1A in human type 2 diabetic islets and effect on insulin secretion publication-title: Diabetologia doi: 10.1007/s00125-007-0916-5 contributor: fullname: Ling – volume: 29 start-page: 2427 year: 2006 ident: 10.1016/j.ymgme.2010.02.004_bib1 article-title: Prevalence and determinants of insulin resistance among US adolescents: a population-based study publication-title: Diabetes Care doi: 10.2337/dc06-0709 contributor: fullname: Lee – volume: 18 start-page: 1427 year: 2002 ident: 10.1016/j.ymgme.2010.02.004_bib15 article-title: MethPrimer: designing primers for methylation PCRs publication-title: Bioinformatics doi: 10.1093/bioinformatics/18.11.1427 contributor: fullname: Li – volume: 339 start-page: 62 year: 2003 ident: 10.1016/j.ymgme.2010.02.004_bib17 article-title: Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data publication-title: Neurosci. Lett. doi: 10.1016/S0304-3940(02)01423-4 contributor: fullname: Ramakers – volume: 25 start-page: 402 year: 2001 ident: 10.1016/j.ymgme.2010.02.004_bib16 article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method publication-title: Methods doi: 10.1006/meth.2001.1262 contributor: fullname: Livak – volume: 2 start-page: S1 year: 2002 ident: 10.1016/j.ymgme.2010.02.004_bib18 article-title: AliBaba2: context specific identification of transcription factor binding sites publication-title: In Silico. Biol. contributor: fullname: Grabe – volume: 19 start-page: 273 year: 2007 ident: 10.1016/j.ymgme.2010.02.004_bib19 article-title: Genomic patterns of DNA methylation: targets and function of an epigenetic mark publication-title: Curr. Opin. Cell Biol. doi: 10.1016/j.ceb.2007.04.011 contributor: fullname: Weber – start-page: 146 year: 1990 ident: 10.1016/j.ymgme.2010.02.004_bib13 article-title: Sample preparation from blood, cells, and other fluids contributor: fullname: Kawasaki – volume: 102 start-page: 401 year: 2008 ident: 10.1016/j.ymgme.2010.02.004_bib4 article-title: Role of mitochondrial dysfunction in insulin resistance publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.107.165472 contributor: fullname: Kim – volume: 8 start-page: 105 year: 2007 ident: 10.1016/j.ymgme.2010.02.004_bib2 article-title: Metabolic programming in the pathogenesis of insulin resistance publication-title: Rev. Endocr. Metab. Disord. doi: 10.1007/s11154-007-9050-4 contributor: fullname: Devaskar – volume: 350 start-page: 664 year: 2004 ident: 10.1016/j.ymgme.2010.02.004_bib5 article-title: Impaired mitochondrial activity in the insulin-resistant offspring of patients with type 2 diabetes publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa031314 contributor: fullname: Petersen – volume: 17 start-page: 1032 year: 2009 ident: 10.1016/j.ymgme.2010.02.004_bib14 article-title: Maternal pregestational BMI is associated with methylation of the PPARGC1A promoter in newborns publication-title: Obesity doi: 10.1038/oby.2008.605 contributor: fullname: Gemma – volume: 39 start-page: 457 year: 2007 ident: 10.1016/j.ymgme.2010.02.004_bib25 article-title: Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome publication-title: Nat. Genet. doi: 10.1038/ng1990 contributor: fullname: Weber – volume: 54 start-page: S3 issue: Suppl. 2 year: 2001 ident: 10.1016/j.ymgme.2010.02.004_bib26 article-title: Mitochondrial transcription factor A (mtTFA) and diabetes publication-title: Diabetes Res. Clin. Pract. doi: 10.1016/S0168-8227(01)00330-8 contributor: fullname: Choi – volume: 9 start-page: 465 year: 2008 ident: 10.1016/j.ymgme.2010.02.004_bib23 article-title: DNA methylation landscapes: provocative insights from epigenomics publication-title: Nat. Rev. Genet. doi: 10.1038/nrg2341 contributor: fullname: Suzuki |
SSID | ssj0011594 |
Score | 2.2121553 |
Snippet | To explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of adolescents... PURPOSETo explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of... Purpose - To explore whether DNA methylation of the mitochondrial transcription factor A (TFAM) promoter is associated with insulin resistance in a sample of... |
SourceID | proquest crossref pubmed elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 83 |
SubjectTerms | Adolescence Adolescent Adolescents Base Sequence DNA Methylation DNA-Binding Proteins - genetics Epigenetics Female Humans Insulin resistance Insulin Resistance - genetics Leukocytes - metabolism Male Metabolic syndrome Metabolic Syndrome - genetics Mitochondrial Proteins - genetics Molecular Sequence Data Promoter Regions, Genetic Sp1 Transcription Factor - metabolism TFAM Transcription Factors - genetics |
Title | Methylation of TFAM gene promoter in peripheral white blood cells is associated with insulin resistance in adolescents |
URI | https://dx.doi.org/10.1016/j.ymgme.2010.02.004 https://www.ncbi.nlm.nih.gov/pubmed/20202876 https://search.proquest.com/docview/733645484 https://search.proquest.com/docview/744693972 |
Volume | 100 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3BTttAEB0hUNVeEAXaBiiaQ9UTbuzEWdvHEBGFVuFSkLit1ruTKqhxIhJAXPh2ZtZeBAc4IFmyZa2t1bzR7pv181uAHz1xLbPdOHLkyii1uYtk47dIpVmcWSa8sZEF_fGZGl2kvy97l2swCP_CiKyyGfvrMd2P1s2ddhPN9mI6bf9NmH1nYucV--9V_g92PnFO_3p4knkw4fGbIUrjSFoH5yGv8bqf_ZtRo-8S4870tdnpNfbpZ6HhFmw29BH7dQ8_wxpV27DTr7h0nt3jT_SCTr9Svg0fjsPVx0HY1m0HbsfE2NQKOJxP8HzYHyNnEeHCK_PoGqcViv-xNxz4j3fyoQG9wB1lmX-J0yWaBlVyKCu52EjakYt3IaQcRXnLM7uoXbgYnpwPRlGz-UJkmUGtIlK9sksxWWY4zAOsykxOmSkyR3x0C8Y-z6g0ie0wpXOJY-qoqHAMSZ6X6aT7BdareUXfAMtEFamaTLiy6aW2JMOMvmOcIcfFmi2oBUch6HpRe2zoID670h4jLRjpuKMZoxaoAIx-kSqaZ4G3H8QAo-agS8hMRfObpRZPyJRrt7eacN1cMHnrtOBrnQFPfeX4MEvL1N57O7YPn4IoIU4OYH11fUPfmeusykOfzIew0T_9Mzp7BFbD_vw |
link.rule.ids | 315,786,790,4521,24144,27957,27958,45620,45714 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pa9swFH6UlNFdxtbuR9ate4ex00zsRJbsYxYW0rXJZSn0JmTrZaQsTmjSjf73fZKlsh3aQ8EHY2Qj3iek7-l9_gTwOXeuZfUgTSzZKhF1YRN38FsihUpVzYQ3NW5DfzqTkwvx4zK_3INR_BfGySrD3N_O6X62Dk96IZq9zXLZ-5kx-1bOziv19SpOgfZFrjLRgf3h6dlkdl9M4BXbF5e5feJeiOZDXuZ1u_q1oiDxct6d4qEF6iEC6hei8Ut4ERgkDttOvoI9ag7haNhw9ry6xS_oNZ1-s_wQnn2LdwejeLLbEfyZEsPTiuBwvcD5eDhFHkiEGy_Oo2tcNugskL3nwG_862oN6DXu6Hb6t7jcognAkkW3mYtB1Y6cvztOyoF0X_nHMeo1XIy_z0eTJJy_kNRMonYJybwaUEo1kxymArVUpiBlSmWJr0HJ8BeKKpPVfWZ1NrPMHiWVllEpikosBm-g06wbegdYZbIUcrHg5CYXdUWGSX3fWEOW87W6pC58jUHXm9ZmQ0f92ZX2GGmHkU77mjHqgozA6P9Gi-aF4PEXMcKoOeguZKah9c1WO1tIwenbY004dS6Zv_W78LYdAfd95fgwUVPy_VM79gkOJvPpuT4_nZ0dw_OoUUizD9DZXd_QR6Y-u-okDO07GaYBwQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Methylation+of+TFAM+gene+promoter+in+peripheral+white+blood+cells+is+associated+with+insulin+resistance+in+adolescents&rft.jtitle=Molecular+genetics+and+metabolism&rft.au=Gemma%2C+Carolina&rft.au=Sookoian%2C+Silvia&rft.au=Dieuzeide%2C+Guillermo&rft.au=Garc%C3%ADa%2C+Silvia+I&rft.date=2010-05-01&rft.eissn=1096-7206&rft.volume=100&rft.issue=1&rft.spage=83&rft.epage=87&rft_id=info:doi/10.1016%2Fj.ymgme.2010.02.004&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1096-7192&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1096-7192&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1096-7192&client=summon |