Genotyping of Essential Hypertension Single-Nucleotide Polymorphisms by a Homogeneous PCR Method with Universal Energy Transfer Primers

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 48; no. 12; pp. 2131 - 2140
• Main Authors: Bengra, Chikh, Mifflin, Theodore E, Khripin, Yuri, Manunta, Paolo, Williams, Scott M, Jose, Pedro A, Felder, Robin A
• Format: Journal Article
• Language: English
• Published: Washington, DC Am Assoc Clin Chem 01.12.2002; American Association for Clinical Chemistry; Oxford University Press
• Subjects: Angiotensinogen - genetics; Biological and medical sciences; Cloning; Cytochrome P-450 CYP11B2 - genetics; Disease; DNA Primers; Energy transfer; Fluorescence Resonance Energy Transfer; Fluorometry; G-Protein-Coupled Receptor Kinase 4; Genes; Genetic screening; Genetic testing; Genomes; Genotype; Genotypes; Humans; Hypertension; Hypertension - genetics; Investigative techniques, diagnostic techniques (general aspects); Kinases; Medical sciences; Methods; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polymerase Chain Reaction - methods; Polymorphism, Single Nucleotide; Protein-Serine-Threonine Kinases - genetics; Human; Hypertension; Primer; Cardiovascular disease; Essential; Vascular disease; Polymerase chain reaction; Cytogenetics; Diagnosis; Single nucleotide polymorphism; Technique; Molecular biology; Energy transfer

Human hypertension is a complex, multifactorial disease with a heritability of more than 30-50%. A genetic screening test based on analysis of multiple single-nucleotide polymorphisms (SNPs) to assess the likelihood of developing hypertension would be helpful for disease management. Tailed allele-sp...