Cross-linking of the dermo-epidermal junction of skin regenerating from keratinocyte autografts. Anchoring fibrils are a target for tissue transglutaminase

• Published in: The Journal of clinical investigation Vol. 98; no. 5; pp. 1174 - 1184
• Main Authors: Raghunath, M, Höpfner, B, Aeschlimann, D, Lüthi, U, Meuli, M, Altermatt, S, Gobet, R, Bruckner-Tuderman, L, Steinmann, B
• Format: Journal Article
• Language: English
• Published: United States 01.09.1996
• Subjects: Adolescent; Amino Acid Sequence; Burns - surgery; Cell Adhesion - physiology; Child; Child, Preschool; Collagen - metabolism; Cross-Linking Reagents; Epidermis - physiology; Extracellular Matrix - physiology; Extracellular Matrix - ultrastructure; Female; Humans; Keratinocytes - transplantation; Keratinocytes - ultrastructure; Male; Microscopy, Immunoelectron; Models, Biological; Molecular Sequence Data; Regeneration; Skin - ultrastructure; Skin Physiological Phenomena; Transglutaminases - immunology; Transglutaminases - isolation & purification; Transglutaminases - physiology; Wound Healing - physiology

Since transglutaminases create covalent gamma-glutamyl-epsilon-lysine cross-links between extracellular matrix proteins they are prime candidates for stabilizing tissue during wound healing. Therefore, we studied the temporo-spatial expression of transglutaminase activity in skin regenerating from c...