Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults

• Published in: World journal of gastroenterology : WJG Vol. 14; no. 13; pp. 2029 - 2036
• Main Authors: Kekkonen, Riina A, Lummela, Netta, Karjalainen, Heli, Latvala, Sinikka, Tynkkynen, Soile, Järvenpää, Salme, Kautiainen, Hannu, Julkunen, Ilkka, Vapaatalo, Heikki, Korpela, Riitta
• Format: Journal Article
• Language: English
• Published: United States University of Helsinki, Institute of Biomedicine, Pharmacology, PO Box 63, 00014 Helsinki, Finland and Valio Ltd, Research Centre, Meijeritie 4, Helsinki 00370, Finland%Valio Ltd, Research Centre, Meijeritie 4, Helsinki 00370, Finland%National Public Health Institute, Department of Viral Diseases and Immunology, Mannerheimintie 166,Helsinki 00300, Finland%Medcare Foundation, Aanekoski, Finland%University of Helsinki, Institute of Biomedicine, Pharmacology, PO Box 63, Helsinki 00014, Finland 07.04.2008; The WJG Press and Baishideng
• Subjects: Adult; Anti-Inflammatory Agents - pharmacology; Area Under Curve; Bifidobacterium - metabolism; C-Reactive Protein - metabolism; Clinical Research; Double-Blind Method; Female; Humans; Immunoglobulins - metabolism; Lactobacillus rhamnosus - metabolism; Leukocytes, Mononuclear - cytology; Male; Middle Aged; Placebos; Probiotics - pharmacology; Propionibacterium - metabolism; Species Specificity; Treatment Outcome; 免疫学; 前生命期; 抗炎效果; 高敏感性C反应蛋白; Probiotic; Highly sensitive C-reactive protein; Inflammation; Immune response; Mononuclear cells; Cytokine
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.14.2029

AIM： To evaluate the effects of three potentially anti-inflammatory probiotic bacteria from three different genera on immune variables in setting based on previous in cytokine responses. healthy adults in a clinical vitro characterization of METHODS： A total of 62 volunteers participated in this randomized, double-blind and placebo-controlled parallel group intervention study. The volunteers were randomized to receive a milk-based drink containing either Lactobacillus rhamnosus GG （LGG）, Bifidobacterium animalis ssp. lactis Bb12 （Bb12）, or Propionibacterium freudenreichii ssp. shermanii JS （PJS） or a placebo drink for 3 wk. Venous blood and saliva samples were taken at baseline and on d 1, 7 and 21. Fecal samples were collected at baseline and at the end of intervention. RESULTS： The serum hsCRP expressed as the median AUC0-21 （minus baseline） was 0.018 mg/L in the placebo group, -0.240 mg/L in the LGG group, 0.090 mg/L in the Bb12 group and -0.085 mg/L in the PJS group （P = 0.014）. In vitro production of TNF-α from in vitro cultured peripheral blood mononuclear cells （PBMC） was significantly lower in subjects receiving LGG vs placebo. IL-2 production from PBMC in the Bb12 group was significantly lower compared with the other groups. CONCLUSION： In conclusion, probiotic bacteria have strain-specific anti-inflammatory effects in healthy adults.