Solid Phase Pegylation of Hemoglobin

• Published in: Artificial cells, blood substitutes, and immobilization biotechnology Vol. 37; no. 4; pp. 147 - 155
• Main Authors: Suo, Xiaoyan, Zheng, Chunyang, Yu, Pengzhan, Lu, Xiuling, Ma, Guanghui, Su, Zhiguo
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.01.2009; Taylor & Francis
• Subjects: Adsorption; Animals; Cattle; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; hemoglobin; Hemoglobins - analysis; Hemoglobins - chemistry; Hemoglobins - isolation & purification; Hemoglobins - metabolism; Indicators and Reagents - chemistry; Lasers; Molecular Weight; polyethylene glycol; Polyethylene Glycols - chemistry; red blood cell substitute; Scattering, Radiation; solid phase

A solid phase conjugation process was developed for attachment of polyethylene glycol to hemoglobin molecule. Bovine hemoglobin was loaded onto an ion exchange chromatography column and adsorbed by the solid medium. Succinimidyl carbonate mPEG was introduced in the mobile phase after the adsorption....