Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system

• Published in: Journal of Asia-Pacific entomology Vol. 18; no. 2; pp. 303 - 309
• Main Authors: Morokuma, Daisuke, Xu, Jian, Mon, Hiroaki, Hirata, Kazuma, Hino, Masato, Kuboe, Shoko, Yamashita, Mami, Kusakabe, Takahiro, Lee, Jae Man
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2015; 한국응용곤충학회
• Subjects: Alpha 1-acid glycoprotein; Baculoviridae; Baculovirus expression vector system; bioactive properties; blood proteins; glycoproteins; glycosylation; hemolymph; humans; larvae; N-glycosylation; polyacrylamide gel electrophoresis; polysaccharides; Silkworm; silkworms; 농수해양학; Baculovirus expression vector system; N-glycosylation; Alpha 1-acid glycoprotein; Silkworm

Glycosylation is an important post-translational modification that confers various biological activities, structural stability, and inter-molecular interactions to proteins. Baculovirus expression vector system (BEVS) is widely used to produce recombinant glycoproteins, which may not be suitable for...