Sonic Hedgehog and FGF8: Inadequate Signals for the Differentiation of a Dopamine Phenotype in Mouse and Human Neurons in Culture

• Published in: Experimental neurology Vol. 169; no. 1; pp. 36 - 43
• Main Authors: Stull, Natalie D., Iacovitti, Lorraine
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.05.2001; Elsevier
• Subjects: 1-Methyl-3-isobutylxanthine - pharmacology; Animals; Biological and medical sciences; Biotechnology; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cells, Cultured; Colforsin - pharmacology; Corpus Striatum - cytology; Corpus Striatum - embryology; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; differentiation; dopamine; Dopamine - metabolism; Dopamine - pharmacology; Dose-Response Relationship, Drug; Drug Synergism; Enzyme Inhibitors - pharmacology; fibroblast growth factor; Fibroblast Growth Factor 8; Fibroblast Growth Factors - pharmacology; Fundamental and applied biological sciences. Psychology; Gene therapy; Health. Pharmaceutical industry; Hedgehog Proteins; Humans; Industrial applications and implications. Economical aspects; MAP Kinase Signaling System - drug effects; Medical sciences; Mice; Neurology; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; NT2 cells; Parkinson's disease; Phenotype; Proteins - pharmacology; Signal Transduction - drug effects; Signal Transduction - physiology; sonic hedgehog; stem cells; striatum; tissue culture; Tissue Plasminogen Activator - pharmacology; Trans-Activators; Tyrosine 3-Monooxygenase - biosynthesis; tyrosine hydroxylase; stem cells; differentiation; fibroblast growth factor; Parkinson's disease; striatum; tissue culture; sonic hedgehog; NT2 cells; dopamine; tyrosine hydroxylase; Cell culture; Stem cell; Parkinson disease; Tyrosine 3-monooxygenase; Signal transduction; Degenerative disease; Protein-tyrosine kinase; Human; Tyrosine; Nervous system diseases; Dopamine; Enzyme; Transferases; Rodentia; Cell differentiation; Gene expression; Cerebral disorder; Vertebrata; Chemotherapy; Phenotype; Mammalia; Treatment; Mouse; Animal; Central nervous system disease; Dopaminergic neuron; Oxidoreductases; Gene therapy; Extrapyramidal syndrome

Embryonic mouse striatal neurons and human neurons derived from the NT2/hNT stem cell line can be induced, in culture, to express the dopaminergic (DA) biosynthetic enzyme tyrosine hydroxylase (TH). The novel expression of TH in these cells is signaled by the synergistic interaction of factors prese...