Genetic Dissection of Tomato Bushy Stunt Virus p19-Protein-Mediated Host-Dependent Symptom Induction and Systemic Invasion

• Published in: Virology (New York, N.Y.) Vol. 266; no. 1; pp. 79 - 87
• Main Authors: Chu, Meihua, Desvoyes, Bénédicte, Turina, Massimo, Noad, Robert, Scholthof, Herman B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.01.2000
• Subjects: Mutagenesis, Site-Directed; Nicotiana - virology; Nicotiana benthamiana; Nicotiana clevelandii; Nicotiana tabacum; p19 gene; p19 protein; Plant Diseases - virology; Plants, Toxic; Spinacia oleracea - virology; Tomato bushy stunt virus; Tombusvirus - genetics; Tombusvirus - metabolism; Tombusvirus - pathogenicity; Transfection; Viral Proteins - genetics; Viral Proteins - metabolism

The plus-sense single-stranded RNA of tomato bushy stunt virus (TBSV) encodes a 19-kDa protein, which is translated from a 3′ proximal open reading frame (p19) that is entirely nested within the cell-to-cell movement gene (p22). Expression of the cytosolic p19-protein induces either a systemic letha...