Lutetium(III)-dependent self-assembly study of ciliate Euplotes octocarinatus centrin

• Published in: Journal of inorganic biochemistry Vol. 102; no. 2; pp. 268 - 277
• Main Authors: Duan, Lian, Zhao, Ya-Qin, Wang, Zhi-Jun, Li, Guo-Ting, Liang, Ai-Hua, Yang, Bin-Sheng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2008
• Subjects: Animals; Calcium - chemistry; Calcium(II); Calcium-Binding Proteins - chemistry; Centrin; EF Hand Motifs; Euplotes - chemistry; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Lutetium - chemistry; Lutetium(III); Naphthalenesulfonates - chemistry; Osmolar Concentration; Protein Structure, Tertiary; Protozoan Proteins - chemistry; Protozoan Proteins - isolation & purification; Self-assembly; Spectrometry, Fluorescence; Temperature; Centrin; Self-assembly; Calcium(II); Lutetium(III)

Ciliate Euplotes octocarinatus centrin (EoCen) is a member of the EF-hand superfamily of calcium-binding proteins, which often associated with the centrosomes and basal bodies. To explore the possible structural role of EoCen, we initiated a physicochemical study of the self-assembly properties of t...