Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitis

• Published in: Tuberculosis (Edinburgh, Scotland) Vol. 112; pp. 27 - 36
• Main Authors: Dhiman, Abhijeet, Haldar, Sagarika, Mishra, Subodh Kumar, Sharma, Neera, Bansal, Anjali, Ahmad, Yusra, Kumar, Amit, Sharma, Tarun Kumar, Tyagi, Jaya Sivaswami
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.09.2018; Elsevier Science Ltd
• Subjects: Affinity; Antibodies; Antigens, Bacterial - cerebrospinal fluid; Antigens, Bacterial - genetics; Aptamer; Aptamer linked immobilized sorbent assay; Aptamers; Aptamers, Nucleotide - chemical synthesis; Aptamers, Nucleotide - genetics; Aptamers, Nucleotide - metabolism; Bacteria; Bacterial Proteins - cerebrospinal fluid; Bacterial Proteins - genetics; Biological evolution; Biomarkers; Biomarkers - cerebrospinal fluid; Case-Control Studies; Cerebrospinal fluid; Deoxyribonucleic acid; Detection; Diagnosis; Diagnostic systems; DNA; Enzyme-linked immunosorbent assay; Humans; Medical diagnosis; Meningitis; Mycobacterium tuberculosis; Mycobacterium tuberculosis - metabolism; Polyclonal antibodies; Predictive Value of Tests; Reagents; Reproducibility of Results; SELEX Aptamer Technique; Tuberculosis; Tuberculosis, Meningeal - cerebrospinal fluid; Tuberculosis, Meningeal - diagnosis; Tuberculosis, Meningeal - microbiology; Tuberculous meningitis; Aptamer; Mycobacterium tuberculosis; Detection; Aptamer linked immobilized sorbent assay; Tuberculous meningitis

Tuberculous meningitis (TBM) is the most severe manifestation of tuberculosis and its diagnosis remains a challenge even today due to the lack of an adequate test. HspX antigen of Mycobacterium tuberculosis was previously established as a reliable diagnostic biomarker for TBM in an ELISA test format...