Monitoring gradient formation in a jet aerated bioreactor
Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (kLa) and can be used for the intensification of mass transfer limited reactions. The jet loop reactor achieves higher kLa values than a stirred tank reactor (STR). The improvement relies on significantly higher local power inp...
Saved in:
Published in | Engineering in life sciences Vol. 19; no. 3; pp. 159 - 167 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
John Wiley and Sons Inc
01.03.2019
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (kLa) and can be used for the intensification of mass transfer limited reactions. The jet loop reactor achieves higher kLa values than a stirred tank reactor (STR). The improvement relies on significantly higher local power inputs (∼104) than those obtainable with the STR. Operation at high local turnover rates requires efficient macromixing, otherwise reactor inhomogeneities might occur. If sufficient homogenization is not achieved, the selectivity of the reaction and the respective yields are decreased. Therefore, the balance between mixing and mass transfer in jet loop reactors is a critical design aspect. Monitoring the dissolved oxygen levels during the turnover of a steady sodium sulfite feed implied the abundance of gradients in the JLR. Prolonged mixing times at identical power input and aeration rates (∼100%) were identified for the JLR in comparison to the STR. The insertion of a draft tube to the JLR led to a more homogenous dissolved oxygen distribution, but unfortunately a reduction of mixing time was not achieved. In case of increased medium viscosities as they may arise in high cell density cultivations, no gradient formation was detected. However, differences in medium viscosity significantly altered the mass transfer and mixing performance of the JLR. |
---|---|
AbstractList | Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (kLa) and can be used for the intensification of mass transfer limited reactions. The jet loop reactor achieves higher kLa values than a stirred tank reactor (STR). The improvement relies on significantly higher local power inputs (∼104) than those obtainable with the STR. Operation at high local turnover rates requires efficient macromixing, otherwise reactor inhomogeneities might occur. If sufficient homogenization is not achieved, the selectivity of the reaction and the respective yields are decreased. Therefore, the balance between mixing and mass transfer in jet loop reactors is a critical design aspect. Monitoring the dissolved oxygen levels during the turnover of a steady sodium sulfite feed implied the abundance of gradients in the JLR. Prolonged mixing times at identical power input and aeration rates (∼100%) were identified for the JLR in comparison to the STR. The insertion of a draft tube to the JLR led to a more homogenous dissolved oxygen distribution, but unfortunately a reduction of mixing time was not achieved. In case of increased medium viscosities as they may arise in high cell density cultivations, no gradient formation was detected. However, differences in medium viscosity significantly altered the mass transfer and mixing performance of the JLR. Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (k L a) and can be used for the intensification of mass transfer limited reactions. The jet loop reactor achieves higher k L a values than a stirred tank reactor (STR). The improvement relies on significantly higher local power inputs (∼10 4 ) than those obtainable with the STR. Operation at high local turnover rates requires efficient macromixing, otherwise reactor inhomogeneities might occur. If sufficient homogenization is not achieved, the selectivity of the reaction and the respective yields are decreased. Therefore, the balance between mixing and mass transfer in jet loop reactors is a critical design aspect. Monitoring the dissolved oxygen levels during the turnover of a steady sodium sulfite feed implied the abundance of gradients in the JLR. Prolonged mixing times at identical power input and aeration rates (∼100%) were identified for the JLR in comparison to the STR. The insertion of a draft tube to the JLR led to a more homogenous dissolved oxygen distribution, but unfortunately a reduction of mixing time was not achieved. In case of increased medium viscosities as they may arise in high cell density cultivations, no gradient formation was detected. However, differences in medium viscosity significantly altered the mass transfer and mixing performance of the JLR. Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (k a) and can be used for the intensification of mass transfer limited reactions. The jet loop reactor achieves higher k a values than a stirred tank reactor (STR). The improvement relies on significantly higher local power inputs (∼10 ) than those obtainable with the STR. Operation at high local turnover rates requires efficient macromixing, otherwise reactor inhomogeneities might occur. If sufficient homogenization is not achieved, the selectivity of the reaction and the respective yields are decreased. Therefore, the balance between mixing and mass transfer in jet loop reactors is a critical design aspect. Monitoring the dissolved oxygen levels during the turnover of a steady sodium sulfite feed implied the abundance of gradients in the JLR. Prolonged mixing times at identical power input and aeration rates (∼100%) were identified for the JLR in comparison to the STR. The insertion of a draft tube to the JLR led to a more homogenous dissolved oxygen distribution, but unfortunately a reduction of mixing time was not achieved. In case of increased medium viscosities as they may arise in high cell density cultivations, no gradient formation was detected. However, differences in medium viscosity significantly altered the mass transfer and mixing performance of the JLR. |
Author | Dietzsch, Christian Weber, Sebastian Kopf, Michael‐Helmut Schaepe, Sebastian Freyer, Stephan |
AuthorAffiliation | 2 Department of Biochemistry/Biotechnology Martin‐Luther‐University Halle‐Wittenberg Halle/Saale Germany 1 Ind. Biotechnology ‐ Chemical and Process Engineering BASF SE Ludwigshafen Germany |
AuthorAffiliation_xml | – name: 2 Department of Biochemistry/Biotechnology Martin‐Luther‐University Halle‐Wittenberg Halle/Saale Germany – name: 1 Ind. Biotechnology ‐ Chemical and Process Engineering BASF SE Ludwigshafen Germany |
Author_xml | – sequence: 1 givenname: Sebastian surname: Weber fullname: Weber, Sebastian organization: Martin‐Luther‐University Halle‐Wittenberg – sequence: 2 givenname: Sebastian surname: Schaepe fullname: Schaepe, Sebastian organization: BASF SE – sequence: 3 givenname: Stephan surname: Freyer fullname: Freyer, Stephan organization: BASF SE – sequence: 4 givenname: Michael‐Helmut surname: Kopf fullname: Kopf, Michael‐Helmut organization: BASF SE – sequence: 5 givenname: Christian surname: Dietzsch fullname: Dietzsch, Christian email: christian.dietzsch@basf.com organization: BASF SE |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32624998$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkEFPGzEQha0KVEjotcdqj70keLyO174gVREFpCAOwNma9c6mRhs7tTet8u-7IWkEJ04z0nzvzdMbsZMQAzH2FfgUOBeX1GU3FRw05yDEJ3YOCvREaFWeHHYuJD9jo5xfBqTSGj6zs1IoIY3R58zcx-D7mHxYFsuEjafQF21MK-x9DIUPBRYv1BdICXtqitrHROgGxQU7bbHL9OUwx-z55_XT_HayeLi5m_9YTJwsZ9VEVpVxJKmtlW6oBW4INZFQqgGQgCUormrkgG2DzghXc2hr56hqS2yAl2N2tfddb-oVNW4ImLCz6-RXmLY2orfvL8H_ssv4xypjzAzkYPD9YJDi7w3l3q58dtR1GChushVScCVAvKLTPepSzDlRe3wD3O76tru-7bHvQfDtbbgj_r_gAZB74K_vaPuBnb1ePM4BdFX-Axzmj04 |
CitedBy_id | crossref_primary_10_35633_inmateh_60_13 crossref_primary_10_1002_aic_17392 |
Cites_doi | 10.1002/bit.20383 10.1002/cite.330601204 10.1017/S0022112082000056 10.1002/0471238961.0209152014090514.a01 10.1021/i200019a028 10.1002/cite.201000098 10.1021/i260061a607 10.1002/bit.260341107 10.1021/i260071a001 10.1016/j.cej.2006.07.002 10.1007/BF00369528 10.1016/S0009-2509(03)00250-1 10.1002/ceat.270110137 10.1002/elsc.201800008 10.1002/cite.330550316 10.1002/cjce.22660 10.1021/op020074q 10.1002/elsc.201700169 10.1002/elsc.201700141 10.1016/j.jbiotec.2011.12.010 10.1002/cite.200900164 10.1002/ceat.270100108 10.1002/aic.690490905 10.1002/(SICI)1097-0290(19991020)65:2<151::AID-BIT4>3.0.CO;2-V |
ContentType | Journal Article |
Copyright | 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. |
Copyright_xml | – notice: 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim – notice: 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. |
DBID | NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1002/elsc.201800122 |
DatabaseName | PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic PubMed CrossRef |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
DocumentTitleAlternate | WEBER et al |
EISSN | 1618-2863 |
EndPage | 167 |
ExternalDocumentID | 10_1002_elsc_201800122 32624998 ELSC1187 |
Genre | technicalNote Journal Article |
GroupedDBID | --- .3N .GA .Y3 05W 0R~ 10A 1L6 1OC 24P 31~ 33P 3SF 3WU 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5GY 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAFWJ AAHBH AAHHS AAONW AAZKR ABCQN ABCUV ABDBF ABEML ABIJN ABJCF ABJNI ACBWZ ACCFJ ACGFS ACPOU ACSCC ACXQS ADBBV ADEOM ADIZJ ADMGS ADZOD AEEZP AEIMD AENEX AEQDE AEUQT AFBPY AFGKR AFKRA AFPKN AFPWT AFZJQ AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR ASPBG ATUGU AUFTA AVWKF AZBYB AZFZN AZVAB BAFTC BBNVY BDRZF BENPR BFHJK BGLVJ BHBCM BHPHI BMNLL BMXJE BNHUX BROTX BRXPI BY8 CCPQU CS3 D-E D-F DCZOG DPXWK DR2 DU5 EBD EBS EJD F00 F01 F04 FEDTE G-S G.N GNP GODZA GROUPED_DOAJ H.T H.X HCIFZ HF~ HHY HVGLF HZ~ IAO IGS IX1 J0M JPC KQQ LAW LC2 LC3 LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES M7P M7S MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ O66 O9- OIG OK1 P2P P2W P2X P4D PIMPY PTHSS Q.N Q11 QB0 R.K ROL RPM RWI RX1 SUPJJ TUS UB1 W8V W99 WBKPD WIH WIK WIN WNSPC WOHZO WQJ WRC WYISQ XG1 XV2 ZZTAW ~IA ~WT ITC NPM AAYXX ACRPL ACYXJ CITATION 7X8 5PM |
ID | FETCH-LOGICAL-c4357-4779ce4efb68def109ea8ee266d1141a31606ba01afdac92cb01fbcce7f3ad103 |
IEDL.DBID | RPM |
ISSN | 1618-0240 |
IngestDate | Tue Sep 17 21:26:52 EDT 2024 Sat Oct 26 05:55:56 EDT 2024 Fri Dec 06 02:33:39 EST 2024 Sat Nov 02 12:21:06 EDT 2024 Sat Aug 24 01:06:24 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Keywords | dissolved oxygen gradient jet loop reactor micromixing mass transfer macromixing |
Language | English |
License | 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c4357-4779ce4efb68def109ea8ee266d1141a31606ba01afdac92cb01fbcce7f3ad103 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://europepmc.org/articles/pmc6999514?pdf=render |
PMID | 32624998 |
PQID | 2420621214 |
PQPubID | 23479 |
PageCount | 9 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_6999514 proquest_miscellaneous_2420621214 crossref_primary_10_1002_elsc_201800122 pubmed_primary_32624998 wiley_primary_10_1002_elsc_201800122_ELSC1187 |
PublicationCentury | 2000 |
PublicationDate | March 2019 |
PublicationDateYYYYMMDD | 2019-03-01 |
PublicationDate_xml | – month: 03 year: 2019 text: March 2019 |
PublicationDecade | 2010 |
PublicationPlace | Germany |
PublicationPlace_xml | – name: Germany – name: Hoboken |
PublicationTitle | Engineering in life sciences |
PublicationTitleAlternate | Eng Life Sci |
PublicationYear | 2019 |
Publisher | John Wiley and Sons Inc |
Publisher_xml | – name: John Wiley and Sons Inc |
References | 1987; 2 1979; 18 1987; 10 2011 2011; 83 2012; 160 1988; 11 2003; 58 1999; 65 2006 2004 2005; 89 1983; 55 2010; 82 1999 2017; 95 2018; 18 1989; 34 2000 1977; 16 2003; 7 1982; 21 1988; 28 2003; 49 1982; 114 1988; 60 2006; 124 e_1_2_10_24_1 e_1_2_10_22_1 e_1_2_10_20_1 Senge B. (e_1_2_10_23_1) 2004 e_1_2_10_2_1 e_1_2_10_4_1 e_1_2_10_18_1 e_1_2_10_3_1 e_1_2_10_19_1 e_1_2_10_6_1 e_1_2_10_16_1 e_1_2_10_5_1 e_1_2_10_17_1 e_1_2_10_8_1 e_1_2_10_14_1 e_1_2_10_7_1 e_1_2_10_15_1 e_1_2_10_12_1 e_1_2_10_9_1 e_1_2_10_13_1 e_1_2_10_10_1 e_1_2_10_11_1 e_1_2_10_30_1 Liepe F. (e_1_2_10_21_1) 1988; 28 e_1_2_10_29_1 e_1_2_10_27_1 e_1_2_10_28_1 e_1_2_10_25_1 e_1_2_10_26_1 |
References_xml | – year: 2011 – volume: 65 start-page: 151 year: 1999 end-page: 159 article-title: Monitoring of genes that respond to process‐related stress in large‐scale bioprocesses publication-title: Biotechnol. Bioeng. – volume: 18 start-page: 281 year: 2018 end-page: 286 article-title: The ejector loop reactor: Application for microbial fermentation and comparison with a stirred‐tank bioreactor publication-title: Eng. Life Sci. – volume: 49 start-page: 2264 year: 2003 end-page: 2282 article-title: Chemical processing and micromixing in confined impinging jets publication-title: AIChE J – volume: 82 start-page: 243 year: 2010 end-page: 250 article-title: Einsatz des Strahlzonen‐Schlaufenreaktors bei stofftransportlimitierten, mehrphasigen chemischen Reaktionen publication-title: Chem. Ing. Tech. – volume: 95 start-page: 180 year: 2017 end-page: 186 article-title: Modelling mass transfer in gas‐liquid two‐phase flow in a jet zone loop reactor publication-title: Can. J. Chem. Eng. – volume: 28 start-page: 381 year: 1988 end-page: 420 article-title: Verfahrenstechnische Berechnungsmethoden: ausrüstungen und ihre berechnung. Stoffvereinigen in fluiden Phasen publication-title: Deutscher Verlag für Grundstoffindustrie – year: 2000 – volume: 60 start-page: 947 year: 1988 end-page: 955 article-title: Makro‐und Mikromischen im Rührkessel publication-title: Chem. Ing. Tech. – volume: 124 start-page: 1 year: 2006 end-page: 5 article-title: Shear rate in stirred tank and bubble column bioreactors publication-title: Chem. Eng. J. – volume: 160 start-page: 3 year: 2012 end-page: 9 article-title: Scale‐up of microbial processes: impacts, tools and open questions publication-title: J. Biotechnol. – volume: 2 start-page: 79 year: 1987 end-page: 94 article-title: Viscous media in tower bioreactors: Hydrodynamic characteristics and mass transfer properties publication-title: Bioprocess Eng – volume: 21 start-page: 706 year: 1982 end-page: 711 article-title: Gas holdups, specific interfacial areas, and mass transfer coefficients of aerated carboxymethyl cellulose solutions in a bubble column publication-title: Ind. Eng. Chem. Process Des. Dev. – volume: 16 start-page: 133 year: 1977 end-page: 137 article-title: Heat transfer in aerated tower filled with non‐Newtonian liquid publication-title: Ind. Eng. Chem. Process Des. Dev. – volume: 18 start-page: 357 year: 1979 end-page: 364 article-title: Review of Measuring Methods and Results in Nonviscous Gas‐Liquid Mass‐Transfer in Stirred Vessels publication-title: Ind. Eng. Chem. Process Des. Dev. – volume: 7 start-page: 471 year: 2003 end-page: 508 article-title: Mixing and the Selectivity of Chemical Reactions publication-title: Org. Process Res. Dev. – volume: 18 start-page: 244 year: 2018 end-page: 253 article-title: Jet aeration as alternative to overcome mass transfer limitation of stirred bioreactors publication-title: Eng. Life Sci. – volume: 58 start-page: 3877 year: 2003 end-page: 3890 article-title: Applying rotary jet heads for mixing and mass transfer in a forced recirculation tank reactor system publication-title: Chem. Eng. Sci. – start-page: 256 year: 2004 end-page: 260 article-title: Rheologische Stoffkennwerte richtig bestimmen publication-title: DMW ‐ Milchwirtsch – volume: 114 start-page: 83 year: 1982 end-page: 103 article-title: Description of mixing with diffusion and reaction in terms of the concept of material surfaces publication-title: J. Fluid Mech. – volume: 55 start-page: 226 year: 1983 end-page: 227 article-title: Einfluß von Stoffeigenschaften auf die Stoffaustauschfläche in Strahldüsenreaktoren publication-title: Chem. Ing. Tech. – year: 2006 – volume: 34 start-page: 1391 year: 1989 end-page: 1392 article-title: On the calculation of shear rate and apparent viscosity in airlift and bubble column bioreactors publication-title: Biotechnol. Bioeng. – volume: 11 start-page: 276 year: 1988 end-page: 287 article-title: The role of energy dissipation in contacting and mixing devices publication-title: Chem. Eng. Technol. – volume: 18 start-page: 579 year: 2018 end-page: 588 article-title: Impact of Nozzle Operation on Mass Transfer in Jet Aerated Loop Reactors. Characterization and Comparison to an Aerated Stirred Tank Reactor publication-title: Eng. Life Sci. – volume: 10 start-page: 56 year: 1987 end-page: 63 article-title: Local energy dissipation in agitated turbulent fluids and its significance for the design of stirring equipment publication-title: Chem Eng Technol – volume: 83 start-page: 349 year: 2011 end-page: 357 article-title: Scale‐down des Strahlzonen‐Schlaufenreaktors: Entwicklung eines Screening‐Tools für transportlimitierte chemische Reaktionen publication-title: Chem. Ing. Tech. – volume: 89 start-page: 453 year: 2005 end-page: 463 article-title: Culture of Escherichia coli under dissolved oxygen gradients simulated in a two‐compartment scale‐down system: Metabolic response and production of recombinant protein publication-title: Biotechnol. Bioeng. – year: 1999 – ident: e_1_2_10_5_1 doi: 10.1002/bit.20383 – ident: e_1_2_10_17_1 doi: 10.1002/cite.330601204 – ident: e_1_2_10_13_1 doi: 10.1017/S0022112082000056 – ident: e_1_2_10_28_1 doi: 10.1002/0471238961.0209152014090514.a01 – ident: e_1_2_10_29_1 doi: 10.1021/i200019a028 – ident: e_1_2_10_4_1 doi: 10.1002/cite.201000098 – ident: e_1_2_10_25_1 doi: 10.1021/i260061a607 – ident: e_1_2_10_24_1 doi: 10.1002/bit.260341107 – ident: e_1_2_10_27_1 doi: 10.1021/i260071a001 – ident: e_1_2_10_26_1 doi: 10.1016/j.cej.2006.07.002 – ident: e_1_2_10_30_1 doi: 10.1007/BF00369528 – ident: e_1_2_10_19_1 – ident: e_1_2_10_20_1 doi: 10.1016/S0009-2509(03)00250-1 – ident: e_1_2_10_11_1 doi: 10.1002/ceat.270110137 – ident: e_1_2_10_8_1 doi: 10.1002/elsc.201800008 – ident: e_1_2_10_18_1 doi: 10.1002/cite.330550316 – ident: e_1_2_10_2_1 doi: 10.1002/cjce.22660 – ident: e_1_2_10_16_1 doi: 10.1021/op020074q – ident: e_1_2_10_10_1 doi: 10.1002/elsc.201700169 – start-page: 256 year: 2004 ident: e_1_2_10_23_1 article-title: Rheologische Stoffkennwerte richtig bestimmen publication-title: DMW ‐ Milchwirtsch contributor: fullname: Senge B. – ident: e_1_2_10_9_1 doi: 10.1002/elsc.201700141 – ident: e_1_2_10_6_1 doi: 10.1016/j.jbiotec.2011.12.010 – ident: e_1_2_10_14_1 – ident: e_1_2_10_3_1 doi: 10.1002/cite.200900164 – ident: e_1_2_10_12_1 doi: 10.1002/ceat.270100108 – ident: e_1_2_10_15_1 doi: 10.1002/aic.690490905 – ident: e_1_2_10_7_1 doi: 10.1002/(SICI)1097-0290(19991020)65:2<151::AID-BIT4>3.0.CO;2-V – ident: e_1_2_10_22_1 – volume: 28 start-page: 381 year: 1988 ident: e_1_2_10_21_1 article-title: Verfahrenstechnische Berechnungsmethoden: ausrüstungen und ihre berechnung. Stoffvereinigen in fluiden Phasen publication-title: Deutscher Verlag für Grundstoffindustrie contributor: fullname: Liepe F. |
SSID | ssj0017881 |
Score | 2.2291806 |
Snippet | Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (kLa) and can be used for the intensification of mass transfer limited reactions. The... Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (k a) and can be used for the intensification of mass transfer limited reactions. The... Jet aerated loop reactors (JLRs) provide high mass transfer coefficients (k L a) and can be used for the intensification of mass transfer limited reactions.... |
SourceID | pubmedcentral proquest crossref pubmed wiley |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 159 |
SubjectTerms | dissolved oxygen gradient jet loop reactor macromixing mass transfer micromixing |
SummonAdditionalLinks | – databaseName: Wiley Online Library - Core collection (SURFmarket) dbid: DR2 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV07T8MwELZQJxh4P8JLRkJichvn7RGVVhUCBqBSt8iPCxSkFLXpwq_nnLShpQNCjEkcy_b5fN_5zp8JuUwigV6D0cxorVggI8lEAiHLAg6ZEcYHsFsD9w9Rrx_cDsLBwin-ih-i3nCzmlGu11bBpZq0vklD0XRYCkKelNEhXIS5H9ucvpvHmj-KW65063FFPGGWzGvO2uh6reXfl63SCtRczZhcRLKlKepuETnvRJWB8t6cFqqpP3_wO_6nl9tkc4ZT6XU1sXbIGuS7ZGOBvXCPiGo9sA_0ZVymjhW0PgxJhzmV9A0KKi1vMxiqhiNEqDZIsE_63c5zu8dmNzEwjXAqZkEcCw0BZCpKDGTcFSATADTuBv0pLn2OfpCSLpeZkVp4Wrk8U1pDnPnScNc_II18lMMRoUIK8DwIfamDAEKtEKFmCCI05wqlFTvkai6J9KMi3EgramUvtYOR1oPhkIu5oFLUCRvokDmMppMUYYcboU3mgUMOK8HVdSFcRY9TJA6Jl0RaF7B828tf8uFrybsdYVNDWycrJfZL89LO3VPbXuZ-_MfyJ2QdX4oq0e2UNIrxFM4Q-RTqvJzdX7Uy_pc priority: 102 providerName: Wiley-Blackwell |
Title | Monitoring gradient formation in a jet aerated bioreactor |
URI | https://onlinelibrary.wiley.com/doi/abs/10.1002%2Felsc.201800122 https://www.ncbi.nlm.nih.gov/pubmed/32624998 https://search.proquest.com/docview/2420621214 https://pubmed.ncbi.nlm.nih.gov/PMC6999514 |
Volume | 19 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LSwMxEB60B9GD-HZ9EUHwlHaz7xylKCIqohZ6W_KY1Ypupbb_38lut7T0IHhcdsmGLxO-b5LJF4CLLJGUNVjDrTGaRypRXGYY8yISWFhpQ0S3NPDwmNz2ort-3F-BuDkLUxXtGz1ol59f7XLwXtVWfn-ZTlMn1nl66CakaojoO6uwSvTbpOjTrQPnj-6yrERk3Bl4NU6NftAhtnGuhSKrNpTWYY20C6UfMlskpSWluVwwOS9kKya62YLNqYRkV3VXt2EFyx3YmDMW3AVZT1X3wN5GVVXXmM3OKbJByRT7wDFTzlIZLdODIYlHt36_B72b69fuLZ9eksANKZ2UR2kqDUZY6CSzWAhfosoQiXctpTpChYJSFK18oQqrjAyM9kWhjcG0CJUVfrgPrXJY4iEwqSQGAcahMlGEsdEEc0H8boTQBGrqwWWDUv5de2HktetxkDto8xm0Hpw3IOYUrm4PQpU4nPzkpAj8hOhSRB4c1KDO2mpGw4N0Ae7ZB84Ke_ENRUhliT2NCA94NTB_dC-_vn_punvWj_79p2NYp-ZkXY12Aq3xaIKnJE_G-oyE-XNwVgXlL4ER5O8 |
link.rule.ids | 230,314,727,780,784,885,1375,27924,27925,46294,46718,53791,53793 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1JS8QwFH64gMtB3K1rBMFTnKZ7jjIoo86IoIK3kuVVR5yO6Pj_fWmng4MHwWNpScOXF77vJS9fAE6yRFLWYA23xmgeqURxmWHMi0hgYaUNEd3SQO826TxG10_x0wzEzVmYqmjf6P5Z-TY4K_svVW3l-8C0mjqx1l2vnZCqIaJvzcJ8HKZSNEn6ePPAOaS7PCsRGXcWXo1Xox-0iG-cb6HIqi2lJVgg9UIJiMymaemX1vxdMvlTylZcdLkKK2MRyc7rzq7BDJbrsPzDWnADZD1Z3QN7_qjqukZsclKR9Uum2CuOmHKmymiZ7g9JProV_E14vLx4aHf4-JoEbkjrpDxKU2kwwkInmcVC-BJVhkjMaynZESoUlKRo5QtVWGVkYLQvCm0MpkWorPDDLZgrhyXuAJNKYhBgHCoTRRgbTUAXxPBGCE2gph6cNijl77UbRl77Hge5gzafQOvBcQNiTgHrdiFUicOvz5w0gZ8QYYrIg-0a1ElbzWh4kE7BPfnAmWFPv6EYqUyxxzHhAa8G5o_u5Rfd-7a7aX333386gsXOQ6-bd69ub_ZgiZqWdW3aPsyNPr7wgMTKSB9WofkNUl7nWw |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1NS-RAEC3cWRA9yH5qdNUWFjy1See7jzI6jK6KoIK30B_VOstOZnDH_7_VyWSYwcOCx5DQaV5XeK-6K68Afpa5pKzBGm6N0TxVueKyxIy7VKCz0iaIfmvg-iYfPqSXj9njUquvpmjf6NFJ_Wd8Uo-em9rK6diEXZ1YeHvdz0nVENGHU-vCD_AxSyjIukR9foDgXdJ9rpWLknsbr86vMYpD4hzvXSjK5lhpA9ZJwVASIstVanqjN9-WTS7L2YaPBp9gay4k2Wk74c-whvUX2FyyF_wKsv1g_QV7emlqu2Zs8bciG9VMsd84Y8obK6NlejQhCel38b_Bw-D8vj_k81YJ3JDeKXhaFNJgik7npUUnIomqRCT2tZTwCJUISlS0ioRyVhkZGx0Jp43BwiXKiij5Dr16UuMOMKkkxjFmiTJpipnRBLYjljdCaAK1COC4Q6mato4YVet9HFce2moBbQBHHYgVBa0_iVA1Tl7_VqQLopxIU6QBbLegLsbqViOAYgXuxQPeEHv1DsVJY4w9j4sAeLMw_5ledX511_fd1nff_aZDWL89G1RXFze_9mCDRpZtedoP6M1eXnGf9MpMHzSR-Q-3dehu |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Monitoring+gradient+formation+in+a+jet+aerated+bioreactor&rft.jtitle=Engineering+in+life+sciences&rft.au=Weber%2C+Sebastian&rft.au=Schaepe%2C+Sebastian&rft.au=Freyer%2C+Stephan&rft.au=Kopf%2C+Michael%E2%80%90Helmut&rft.date=2019-03-01&rft.pub=John+Wiley+and+Sons+Inc&rft.issn=1618-0240&rft.eissn=1618-2863&rft.volume=19&rft.issue=3&rft.spage=159&rft.epage=167&rft_id=info:doi/10.1002%2Felsc.201800122&rft_id=info%3Apmid%2F32624998&rft.externalDBID=PMC6999514 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1618-0240&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1618-0240&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1618-0240&client=summon |