First report in South America of companion animal colonization by the USA1100 clone of community-acquired meticillin-resistant Staphylococcus aureus (ST30) and by the European clone of methicillin-resistant Staphylococcus pseudintermedius (ST71)

• Published in: BMC research notes Vol. 6; no. 1; p. 336
• Main Authors: Quitoco, Isidório Mebinda Zuco, Ramundo, Mariana Severo, Silva-Carvalho, Maria Cícera, Souza, Raquel Rodrigues, Beltrame, Cristiana Ossaille, de Oliveira, Táya Figueiredo, Araújo, Rodrigo, Del Peloso, Pedro Fernandez, Coelho, Leonardo Rocchetto, Figueiredo, Agnes Marie Sá
• Format: Journal Article
• Language: English
• Published: England BioMed Central 27.08.2013
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Bacterial Proteins - genetics; Brazil - epidemiology; Carrier State; Cat Diseases - epidemiology; Cat Diseases - microbiology; Cats; Cloning; Dog Diseases - epidemiology; Dog Diseases - microbiology; Dogs; Genes; Health care; Hospitals, Animal; Humans; Methicillin-Resistant Staphylococcus aureus - drug effects; Methicillin-Resistant Staphylococcus aureus - genetics; Methicillin-Resistant Staphylococcus aureus - isolation & purification; Microbial Sensitivity Tests; Microbiology; Penicillin-Binding Proteins; Pets; Polymerase chain reaction; Staphylococcal Infections - epidemiology; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcus - drug effects; Staphylococcus - genetics; Staphylococcus - isolation & purification; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Staphylococcus infections; Staphylococcus saprophyticus; Urogenital system; Brazil
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/1756-0500-6-336

Methicillin-resistant staphylococci can colonize and cause diseases in companion animals. Unfortunately, few molecular studies have been carried out in Brazil and other countries with the aim of characterizing these isolates. Consequently, little is known about the potential role of companion animals in transmitting these resistant bacteria to humans. In this work we searched for mecA gene among Staphylococcus isolates obtained from nasal microbiota of 130 healthy dogs and cats attended in a veterinary clinic located in the west region of Rio de Janeiro. The isolates recovered were identified to the species level and characterized using molecular tools. A community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolate related to USA1100 (Southwest Pacific clone) and susceptible to all non-β-lactams was detected in a cat (1.7%, 1/60). Another coagulase-positive isolate harboring mecA was recovered from a dog (1.4%, 1/70) and identified as Staphylococcus pseudintermedius (MRSP) related to the European clone (ST71). The two isolates of Staphylococcus conhii subsp. urealyticus (1.4%, 1/70 dogs and 1.7%, 1/60 cats), similarly to the MRSP isolate, also presented high-level multiresistance. The majority of the methicillin-resistant coagulase-negative staphylococci recovered were Staphylococcus saprophyticus (5.7%, 4/70 dogs and 6.7%, 4/60 cats) and all clustered into the same PFGE type. This work demonstrates that mecA-harboring Staphylococcus isolates are common members of the nasal microbiota of the healthy companion animals studied (9.2%, 12/130 animals), including some high-level multiresistant isolates of S. pseudintermedius and S. conhii subsp. urealyticus. The detection, for the first time in South America, of USA1100-related CA-MRSA and of ST71 MRSP (European clone), colonizing companion animals, is of concern. Both S. pseudintermedius and S. aureus are important agents of infections for animals. The USA1100 CA-MRSA is a causative of severe and disseminated diseases in healthy children and adults. Additionally, MRSP is a nosocomial pathogen in veterinarian settings. It had already been demonstrated that the virulent ST71 MRSP is geographically spread over Europe and USA, with potential for zoonotic infections.