Sequence analyses and evolutionary relationships among the energy‐coupling proteins enzyme I and HPr of the bacterial phosphoenolpyruvate: Sugar phosphotransferase system

• Published in: Protein science Vol. 2; no. 4; pp. 506 - 521
• Main Authors: Reizer, Jonathan, Hoischen, Christian, Reizer, Aiala, Pham, Tam N., Saier, Milton H.
• Format: Journal Article
• Language: English
• Published: Bristol Cold Spring Harbor Laboratory Press 01.04.1993
• Subjects: Amino Acid Sequence; Bacillus subtilis; Bacillus subtilis - enzymology; Bacillus subtilis - genetics; Base Sequence; Biological Evolution; Codon - genetics; codon usage; Cross Reactions; DNA, Bacterial - genetics; Enzyme I; Escherichia coli - enzymology; Escherichia coli - genetics; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; HPr; Molecular Sequence Data; Nucleic Acid Conformation; Open Reading Frames; Operon; Phosphoenolpyruvate Sugar Phosphotransferase System - genetics; Phosphoenolpyruvate Sugar Phosphotransferase System - immunology; phosphotransferase system; phylogenetic trees; Q‐linkers; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; RNA, Messenger - chemistry; RNA, Messenger - genetics; Sequence Homology, Amino Acid

We have previously reported the overexpression, purification, and biochemical properties of the Bacillus subtilis Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) (Reizer, J., et al., 1992, J. Biol. Chem. 267, 9158–9169). We now report the sequencing of the ptsl gene of B. s...