Intravenous injection of Xuebijing attenuates acute kidney injury in rats with paraquat intoxication

• Published in: World journal of emergency medicine Vol. 8; no. 1; pp. 61 - 64
• Main Authors: Xu, Jia-Jun, Zhen, Jian-Tao, Tang, Li, Lin, Qing-Ming
• Format: Journal Article
• Language: English
• Published: China World Journal of Emergency Medicine (WJEM) 2017; Second Affiliated Hospital of Zhejiang University School of Medicine
• Subjects: Edema; Kidneys; Laboratory animals; Original; Pathology; Poisoning; Rodents; Sepsis; Tumor necrosis factor-TNF; United States > US; China; Paraquat; Inflammation; Kidney injury; Xuebijing
• ISSN: 1920-8642
• DOI: 10.5847/wjem.j.1920-8642.2017.01.011

BACKGROUND: The study aimed to investigate the therapeutic benefits of intravenous Xuebijing on acute kidney injury(AKI) in rats with paraquat intoxication.METHODS: Male Sprague-Dawley rats were randomly divided equally into three groups: sham group(n=8), paraquat group(n=8) and Xuebijing-treated group(n=8) using a random number table. The rats were intraperitoneally injected with 50 mg/kg of paraquat. One hour after paraquat administration, the rats were treated intravenously with Xuebijing(8 mL /kg). At 12 hours after paraquat administration, serum was collected to evaluate kidney function, then the rats were sacrificed and kidney samples were immediately harvested. AKI scores were evaluated by renal histopathology and pro-in? ammatory cytokines mR NA levels in kidney were assayed using real-time RT-PCR.RESULTS: Serum urea nitrogen, creatinine and AKI scores were significantly higher in the paraquat group, compared with the sham group(P<0.05, respectively). Moreover, interleukin(IL)-1β, IL-6 and TNF-α m RNA levels were signi? cantly higher in the paraquat group(P<0.01, respectively). However, intravenous Xuebijing signi? cantly decreased serum urea nitrogen, creatinine, AKI scores and IL-1β, IL-6 and TNF-α m RNA levels, compared with the paraquat group(P<0.05, respectively).CONCLUSION: Intravenous Xuebijing attenuates AKI following paraquat poisoning by suppressing in? ammatory response.