Reconstructing the free-energy landscape of a polyprotein by single-molecule experiments

• Published in: Europhysics letters Vol. 82; no. 5; pp. 58006 - 58006 (5)
• Main Authors: Imparato, A, Sbrana, F, Vassalli, M
• Format: Journal Article
• Language: English
• Published: IOP Publishing 01.06.2008; EDP Sciences
• Subjects: 05.70.Ln; 82.37.Rs; 87.15.La

The mechanical unfolding of an engineered protein composed of eight domains of Ig27 is investigated by using atomic force microscopy. Exploiting a fluctuation relation, the equilibrium free energy as a function of the molecule elongation is estimated from pulling experiments. Such a free energy exhi...