Determination of iodoform in rabbit plasma by HPLC

• Published in: BUNSEKI KAGAKU Vol. 42; no. 4; pp. 215 - 218
• Main Authors: NAGATA, Etsuko, YASUDA, Shuuichi, KASAHARA, Tomoyuki, KUBO, Hiroaki, MIURA, Sadanori
• Format: Journal Article
• Language: Japanese; English
• Published: Tokyo The Japan Society for Analytical Chemistry 1993; Nippon Bunseki Kagakkai; Japan Science and Technology Agency
• Subjects: drug monitoring; HPLC; iodoform intoxication; plasma iodoform concentration; plasma of rabbit
• ISSN: 0525-1931
• DOI: 10.2116/bunsekikagaku.42.4_215

A simple, rapid and reproducible method for the determination of iodoform in rabbit plasma by HPLC has been developed. Under the light-resistant condition, iodoform and diiodomethane (as internal standard) in 1.0 ml plasma were extracted with 1.0 ml diethylether. Twenty microliters of the organic phase were injected into the HPLC system, consisted of a Model 510, U6K-injector, Model 481 UV detector setting at 336 nm (0.005 AUFS), an Ultrasphere ODS 5 μm guard column (45 mm × 4.6 mm i.d. ) and an analytical column (150 mm ×4.6 mm i.d.). The mobile phase was 60% methanol / pH 3.8, 5 mM potassium phosphate buffer. The flow rate was 1.0 ml/min. The retention time for iodoform and diiodomethane were 10.4 and 7.7 min, respectively. The limit of detection was 0.3 μg/ml (S/N=3) of plasma. Within-run reproducibility for 0.4 μg/ml was ± 2.1% (n=9). The HPLC separation can be completed in less than 11 min and had been applied for plasma of rabbit packed intraperitoneally by iodoform gauze.