Purification, antioxidant and hepatoprotective activities of polysaccharide from Cissus pteroclada Hayata

Polysaccharide of Cissus pteroclada Hayata (CPHP) was extracted and purified. Three major fractions (CPHP I, CPHP II-1 and CPHP II-2) from the CPHP were purified by column chromatography and investigated for their monosaccharide compositions, scavenging radical effects and hepatoprotective activitie...

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Bibliographic Details
Published inInternational journal of biological macromolecules Vol. 77; pp. 307 - 313
Main Authors Li, Yi-jie, Lin, Dan-dan, Jiao, Bing, Xu, Cheng-ting, Qin, Jiang-ke, Ye, Gao-jie, Su, Gui-fa
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.2015
Subjects
Antioxidant activity
Cell Line
Cell Survival - drug effects
Cissus - chemistry
Cissus pteroclada Hayata
Cytoprotection - drug effects
Free Radical Scavengers - chemistry
Free Radical Scavengers - isolation & purification
Free Radical Scavengers - pharmacology
Hepatoprotective activity
Humans
Hydrogen Peroxide - adverse effects
Liver - cytology
Liver - drug effects
Polysaccharide
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Hepatoprotective activity
Cissus pteroclada Hayata
Polysaccharide
Antioxidant activity
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Summary:Polysaccharide of Cissus pteroclada Hayata (CPHP) was extracted and purified. Three major fractions (CPHP I, CPHP II-1 and CPHP II-2) from the CPHP were purified by column chromatography and investigated for their monosaccharide compositions, scavenging radical effects and hepatoprotective activities in vitro. The results showed that glucose and galactose were the main monosaccharides of three polysaccharide fractions, CPHP II-1 and CPHP II-2 were acidic polysaccharide fractions which contained glucuronic acid and galacturonic acid. Antioxidant activity determination suggested that CPHP I and CPHP II-1 had a higher scavenging effects on DPPH, superoxide radical, hydroxyl radical and ABTS radical. And the results of antioxidant test in vitro showed that CPHP II-2 could significantly increase (P<0.01) the activities of SOD and GSH-Px and decreased MDA level in human hepatocyte cell line (HL7702 cell), which indicating that CPHP II-2 possessed good hepatoprotective activity.
Bibliography:ObjectType-Article-1
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content type line 23
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2015.03.039