Engineering of Corynebacterium glutamicum to Enhance L-ornithine Production by Gene Knockout and Comparative Proteomic Analysis

• Published in: Chinese journal of chemical engineering Vol. 20; no. 4; pp. 731 - 739
• Main Author: 卢冬梅 刘建忠 毛宗万
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2012
• Subjects: 2-oxoglutarate dehydrogenase; Corynebacterium glutamicum; Enzymes; gene knockout; Genes; Glutamates; Inactivation; L-ornithine production; L-鸟氨酸; Pathways; Phosphates; proteomic analysis; Strain; 基因敲除; 学分; 工程菌; 比较蛋白质组; 生产; 生物合成途径; 谷氨酸棒杆菌; gene knockout; L-ornithine production; 2-oxoglutarate dehydrogenase; Corynebacterium glutamicum; proteomic analysis

Engineered Corynebacterium glutamicum was constructed for L-ornithine production by disrupting genes of argF and proB to prevent the flux away from L-ornithine.Effect of the inactivation of 2-oxoglutarate de-hydrogenase complex（ODHC） on L-ornithine production was also investigated.It was found that...