Valuation of brewer's spent grain using a fully recyclable integrated process for extraction of proteins and arabinoxylans

•A sequential extraction of proteins and arabinoxylans from BSG with increasing alkali strength was performed.•A ratio of 1:2 (w/v) at room temperature for 24h saves reagents and energy.•A yield of 82–85% of the BSG total proteins and 66–73% of total AX were obtained.•Alkaline extracts were acidifie...

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Published inIndustrial crops and products Vol. 52; pp. 136 - 143
Main Authors Vieira, Elsa, Rocha, M. Angélica M., Coelho, Elisabete, Pinho, Olívia, Saraiva, Jorge A., Ferreira, Isabel M.P.L.V.O., Coimbra, Manuel A.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.01.2014
Subjects
Online AccessGet full text
ISSN0926-6690
1872-633X
DOI10.1016/j.indcrop.2013.10.012

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Abstract •A sequential extraction of proteins and arabinoxylans from BSG with increasing alkali strength was performed.•A ratio of 1:2 (w/v) at room temperature for 24h saves reagents and energy.•A yield of 82–85% of the BSG total proteins and 66–73% of total AX were obtained.•Alkaline extracts were acidified to pH 3 with citric acid, to obtain the protein-rich fractions.•The AX were recovered by ethanol precipitation and citric acid and ethanol were recycled. The objective of this work was to design an integrated process to valuate brewer's spent grain (BSG) proteins and arabinoxylans (AX) to be used as food ingredients. For this purpose, a sequential extraction of proteins and AX from BSG with increasing alkali (KOH or NaOH) concentrations of 0.1M, 0.5M, and 4M, was optimized. A ratio of 1:2 (w/v) (weight of BSG by volume of alkali solution) at room temperature for 24h was preferred to minimize reagents and energy consumption. To fully integrate the process, alkaline extracts were acidified to pH3 with citric acid, to obtain the protein-rich fractions. The AX were recovered by ethanol precipitation and citric acid and ethanol were recycled. This integrated extraction process allowed a yield of 82–85% of the BSG total proteins and 66–73% of total AX with formation of a cellulose rich residue almost devoid of nitrogen.
AbstractList The objective of this work was to design an integrated process to valuate brewer's spent grain (BSG) proteins and arabinoxylans (AX) to be used as food ingredients. For this purpose, a sequential extraction of proteins and AX from BSG with increasing alkali (KOH or NaOH) concentrations of 0.1M, 0.5M, and 4M, was optimized. A ratio of 1:2 (w/v) (weight of BSG by volume of alkali solution) at room temperature for 24h was preferred to minimize reagents and energy consumption. To fully integrate the process, alkaline extracts were acidified to pH3 with citric acid, to obtain the protein-rich fractions. The AX were recovered by ethanol precipitation and citric acid and ethanol were recycled. This integrated extraction process allowed a yield of 82–85% of the BSG total proteins and 66–73% of total AX with formation of a cellulose rich residue almost devoid of nitrogen.
•A sequential extraction of proteins and arabinoxylans from BSG with increasing alkali strength was performed.•A ratio of 1:2 (w/v) at room temperature for 24h saves reagents and energy.•A yield of 82–85% of the BSG total proteins and 66–73% of total AX were obtained.•Alkaline extracts were acidified to pH 3 with citric acid, to obtain the protein-rich fractions.•The AX were recovered by ethanol precipitation and citric acid and ethanol were recycled. The objective of this work was to design an integrated process to valuate brewer's spent grain (BSG) proteins and arabinoxylans (AX) to be used as food ingredients. For this purpose, a sequential extraction of proteins and AX from BSG with increasing alkali (KOH or NaOH) concentrations of 0.1M, 0.5M, and 4M, was optimized. A ratio of 1:2 (w/v) (weight of BSG by volume of alkali solution) at room temperature for 24h was preferred to minimize reagents and energy consumption. To fully integrate the process, alkaline extracts were acidified to pH3 with citric acid, to obtain the protein-rich fractions. The AX were recovered by ethanol precipitation and citric acid and ethanol were recycled. This integrated extraction process allowed a yield of 82–85% of the BSG total proteins and 66–73% of total AX with formation of a cellulose rich residue almost devoid of nitrogen.
Author Pinho, Olívia
Coelho, Elisabete
Saraiva, Jorge A.
Ferreira, Isabel M.P.L.V.O.
Rocha, M. Angélica M.
Coimbra, Manuel A.
Vieira, Elsa
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  givenname: M. Angélica M.
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  givenname: Elisabete
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  organization: QOPNA, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal
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  givenname: Olívia
  surname: Pinho
  fullname: Pinho, Olívia
  organization: REQUIMTE – Deapartamento de Ciências Químicas, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, 450-313 Porto, Portugal
– sequence: 5
  givenname: Jorge A.
  surname: Saraiva
  fullname: Saraiva, Jorge A.
  organization: QOPNA, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal
– sequence: 6
  givenname: Isabel M.P.L.V.O.
  surname: Ferreira
  fullname: Ferreira, Isabel M.P.L.V.O.
  organization: REQUIMTE – Deapartamento de Ciências Químicas, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira 228, 450-313 Porto, Portugal
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  givenname: Manuel A.
  orcidid: 0000-0001-8898-6342
  surname: Coimbra
  fullname: Coimbra, Manuel A.
  email: mac@ua.pt
  organization: QOPNA, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal
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Snippet •A sequential extraction of proteins and arabinoxylans from BSG with increasing alkali strength was performed.•A ratio of 1:2 (w/v) at room temperature for 24h...
The objective of this work was to design an integrated process to valuate brewer's spent grain (BSG) proteins and arabinoxylans (AX) to be used as food...
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SubjectTerms ambient temperature
arabinoxylan
Arabinoxylans
Brewer's spent grain
cellulose
citric acid
energy
ethanol
ingredients
nitrogen
potassium hydroxide
Proteins
Recycling
sodium hydroxide
spent grains
Title Valuation of brewer's spent grain using a fully recyclable integrated process for extraction of proteins and arabinoxylans
URI https://dx.doi.org/10.1016/j.indcrop.2013.10.012
https://www.proquest.com/docview/1836656431
Volume 52
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