High-sensitive electrochemical sensor for determination of Norfloxacin and its metabolism using MWCNT-CPE/pRGO-ANSA/Au

• Published in: Sensors and actuators. B, Chemical Vol. 257; pp. 1065 - 1075
• Main Authors: Liu, Zhenping, Jin, Mingliang, Cao, Jieping, Wang, Juan, Wang, Xin, Zhou, Guofu, van den Berg, Albert, Shui, Lingling
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.03.2018; Elsevier Science Ltd
• Subjects: Carbon; Chemical sensors; Electrical resistivity; Electrochemical sensor; Electrodes; Formulations; Gold; Gold nanoparticles; Graphene oxide; Liquid chromatography; Metabolism; Multi wall carbon nanotubes; Multi-walled carbon nanotubes; Nanomaterials; Nanotubes; Norfloxacin; Pharmaceuticals; Sensors; Voltammetry; Graphene oxide; Electrochemical sensor; Norfloxacin; Multi-walled carbon nanotubes; Metabolism; Gold nanoparticles
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2017.11.052

A composite electrode of MWCNT-CPE/pRGO-ANSA/Au has been fabricated for electrochemical determination of Norfloxacin with the detection limit of 0.016μM (S/N=3), being successfully applied for NFX determination in pharmaceutical formulations and rat plasma samples. [Display omitted] •The composite MWCNT-CPE/pRGO-ANSA/Au electrochemical sensor was fabricated.•Fabricated sensors showed low detection limit, good selectivity and stability for NFX detection.•NFX was precisely assayed using the modified electrode in various samples. In this work, a high-sensitive electrochemical sensor of Norfloxacin (NFX) has been developed using a composite electrode of MWCNT-CPE/pRGO-ANSA/Au. MWCNT-CPE is the multi-walled carbon nanotubes (MWCNT) – carbon paste electrode (CPE). pRGO-ANSA means the partially reduced graphene oxide (pRGO) – 6-aminonaphthalene-2-sulphonic acid (ANSA) hybrid nanomaterials. Au represents gold nanoparticles. The electrochemical sensing of NFX was investigated by cyclic voltammetry (CV), linear sweep voltammetry (LSV) and differential pulse voltammetry (DPV). Experimental results show that the combination of MWCNT-CPE/pRGO-ANSA/Au offers high electrical conductivity, large surface area and excellent electrocatalytic activity, being beneficial for electrochemical sensing performance. The calibration curves of NFX exhibit linear response in the NFX concentration range of 0.03–1.0 and 1.0–50.0μM, with the limit of detection (LOD) of 0.016μM (S/N=3). The fabricated sensors were successfully applied for detecting the presence of NFX in pharmaceutical formulations and rat plasma samples. The measured results were comparable with those obtained using ultrahigh performance liquid chromatography (UHPLC), indicating its high potential for applications in pharmaceutical analysis and clinical therapeutic drug monitoring.