Probing the binding of trypsin to glutathione-stabilized gold nanoparticles in aqueous solution

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 135; pp. 261 - 266
• Main Authors: Wang, Gongke, Liu, Xingbing, Yan, Changling, Bai, Guangyue, Lu, Yan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2015
• Subjects: Agglomeration; Au nanoparticles; Binding; Constants; Cooperative binding; Fluorescence; Fluorescence quenching; Glutathione - chemistry; Gold; Gold - chemistry; Interaction; Metal Nanoparticles - chemistry; Nanoparticles; Protein Binding; Protein Conformation; Solutions; Thermodynamics; Trypsin; Trypsin - chemistry; Ultraviolet; Water; Au nanoparticles; Trypsin; Cooperative binding; Interaction; Fluorescence quenching

We investigate the interaction of trypsin with glutathione-stabilized Au nanoparticles (NPs) using fluorescence, synchronous fluorescence and ultraviolet (UV) absorption spectroscopy. We find that trypsin binds strongly to the Au NPs with a static quenching mechanism, and that the interaction is cha...