Evaluation of long-term preservation methods for viral RNA in mosquitoes at room temperature

• Published in: Journal of virological methods Vol. 325; p. 114887
• Main Authors: Kai, Izumi, Kobayashi, Daisuke, Itokawa, Kentaro, Sanjoba, Chizu, Itoyama, Kyo, Isawa, Haruhiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2024
• Subjects: Aedes; Animals; Culicidae; Mosquito Vectors; Mosquito-borne viruses; Mosquitoes; Nucleic acid preservation; Reagents; RNA viruses; RNA, Viral - genetics; Room temperature; Temperature; Mosquito-borne viruses; RNA viruses; nt; PVY; Mosquitoes; EVE; RT-PCR; DENV-1; Reagents; SGM; Nucleic acid preservation; NGS; RT-qPCR; Room temperature; AealbAV

Mosquitoes are important vectors of various pathogenic viruses. Almost all viruses transmitted by mosquitoes are RNA viruses. Therefore, to detect viral genes, mosquito samples must be kept at low temperatures to prevent RNA degradation. However, prolonged transport from the field to laboratory can...