Tissue Handling and Specimen Preparation in Surgical Pathology: Issues Concerning the Recovery of Nucleic Acids From Formalin-Fixed, Paraffin-Embedded Tissue

• Published in: Archives of pathology & laboratory medicine (1976) Vol. 132; no. 12; pp. 1929 - 1935
• Main Authors: Hewitt, Stephen M., Lewis, Fraser A., Cao, Yanxiang, Conrad, Richard C., Cronin, Maureen, Danenberg, Kathleen D., Goralski, Thomas J., Langmore, John P., Raja, Rajiv G., Williams, P. Mickey, Palma, John F., Warrington, Janet A.
• Format: Journal Article
• Language: English
• Published: Northfield College of American Pathologists 01.12.2008
• Subjects: Acids; Biomarkers; Clinical trials; Criminal investigation; Histopathology; Ischemia; Laboratories; Medical research; Medicine, Experimental; Pathology; Quality standards; RNA; Standardization

Context.—Expression profiling by microarrays and real-time polymerase chain reaction–based assays is a powerful tool for classification and prognostication of disease; however, it remains a research tool, largely reliant on frozen tissue. Limiting the utility of expression profiling is the isolation...