Flavone-8-acetic acid (flavonoid) profoundly reduces platelet-dependent thrombosis and vasoconstriction after deep arterial injury in vivo

• Published in: Circulation (New York, N.Y.) Vol. 101; no. 3; pp. 324 - 328
• Main Authors: MRUK, J. S, WEBSTER, M. W. I, HERAS, M, REID, J. M, GRILL, D. E, CHESEBRO, J. H
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 25.01.2000; American Heart Association, Inc
• Subjects: Animals; Antineoplastic Agents - pharmacology; Biological and medical sciences; Blood Platelets - drug effects; Blood. Blood coagulation. Reticuloendothelial system; Catheterization; Flavonoids - pharmacokinetics; Flavonoids - pharmacology; Medical sciences; Pharmacology. Drug treatments; Platelet Glycoprotein GPIb-IX Complex - antagonists & inhibitors; Swine; Thrombosis - drug therapy; Vasoconstriction - drug effects; Vasoconstriction; Instrumentation therapy; Cardiovascular disease; Instrumental dilatation; Flavonoid; Thrombosis; Artery; Antiplatelet agent; Pig; Arterial disease; Vascular disease; Aggregation; Prevention; Restenosis; Vertebrata; Chemotherapy; Platelet; Mammalia; Treatment; Animal; Complication; Artiodactyla; Ungulata
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/01.CIR.101.3.324

Flavone-8-acetic acid (FAA; [Flavonoid]), an adjuvant antitumor drug, inhibits ristocetin-induced aggregation of human platelets. The effect of FAA on platelet-dependent thrombosis was studied in vivo in the porcine carotid artery after deep arterial injury by balloon angioplasty. (111)In-labeled autologous platelet and (125)I-labeled porcine fibrin(ogen) deposition, and the incidence of macroscopic mural thrombosis onto deeply injured artery (tunica media) were compared in 20 pigs (40+/-1 kg [mean+/-SEM], body surface area=1.0+/-0.1 m(2)), randomized to FAA bolus (n=10) of 5.5g/m(2), followed by an infusion at 0.14g. m(-2). min(-1) or placebo (n=10). Vasoconstriction was measured immediately beyond the dilated segment using quantitative angiography. Platelet deposition (x10(6)/cm(2) of carotid artery) was reduced over 12-fold in pigs treated with FAA (13+/-3 versus 164+/-51, P=0.001) compared with placebo. Fibrin(ogen) deposition (x10(12) molecules/cm(2) of carotid artery) did not significantly differ in FAA-treated pigs versus placebo (40+/-8 versus 140+/-69, P=0.08). Large mural thrombi were present in 100% of placebo-treated pigs versus very small thrombi in 40% of FAA-treated pigs (P=0.005). Vasoconstriction was reduced from 46+/-6% in the placebo group to 15+/-3% in the FAA group (P<0.001). Plasma level of FAA before angioplasty was 515+/-23 microgram/mL. The activated partial thromboplastin time was unchanged. The bleeding time was >2SD above the normal mean in 4 of 5 treated pigs (increased from 157+/-29 to 522+/-123 s). FAA markedly reduced platelet deposition, mural thrombi, and injury-induced vasoconstriction after deep arterial injury, suggesting that a major inhibition of platelet glycoprotein Ibalpha may be beneficial therapy.