Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes

• Published in: International journal of biological macromolecules Vol. 93; no. Pt A; pp. 136 - 144
• Main Authors: Zhao, Yong-ming, Wang, Jin, Wu, Zhi-gang, Yang, Jian-ming, Li, Wei, Shen, Li-xia
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2016
• Subjects: Animals; Anti- proliferative activity; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Cell Proliferation - drug effects; Cellulase - chemistry; Drug Screening Assays, Antitumor; Enzyme-assisted extraction; Fungal Polysaccharides - isolation & purification; Fungal Polysaccharides - pharmacology; HCT116 Cells; HeLa Cells; Humans; Hydrogen-Ion Concentration; Lentinus edodes polysaccharides; Liquid-Liquid Extraction; Mice; Neoplasm Transplantation; Papain - chemistry; Polygalacturonase - chemistry; Sarcoma - drug therapy; Shiitake Mushrooms - chemistry; Enzyme-assisted extraction; Lentinus edodes polysaccharides; Anti- proliferative activity
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.05.100

In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated. An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively. Then effects of extraction conditions were evaluated and optimized using a Box-Behnken design. The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%. Subsequently, the crude LEPs were further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions, which were designated as LEP-1 and LEP-2 and their monosaccharide compositions were characterized by GC. Fourier-transform infrared spectra demonstrated that LEP-1 and LEP-2 were distinct from each other regarding their chemical structures. In addition, the LEPs exhibited inhibition of cell proliferation on HCT-116 and HeLa cells in vitro. In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.