Microextraction method of medium and long chain fatty acids from milk

• Published in: Food chemistry Vol. 172; pp. 456 - 461
• Main Authors: Añorve-Morga, Javier, Castañeda-Ovando, Araceli, Cepeda-Saez, Alberto, Archibold, Armando Durant, Jaimez-Ordaz, Judith, Contreras-López, Elizabeth, González-Olivares, Luis Guillermo, Rodríguez-Rodríguez, José Luis
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2015
• Subjects: Animals; Chromatography, Gas; Fatty acid; Fatty Acids - analysis; Fatty Acids, Monounsaturated - analysis; Fatty Acids, Omega-3 - analysis; Fatty Acids, Omega-6 - analysis; Microextraction method; Milk - chemistry; Milk fat; Omega-3; Reproducibility of Results; Microextraction method; Omega-3; Milk fat; Fatty acid
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2014.09.048

•A simple method for extracting medium and long chain milk fatty acids was proposed.•The proposed method used a lower amount of sample and solvents.•The chromatographic method is reproducible and repeatable.•The percentage of recovery obtained is higher compared with the standard method. The aim of this research was to develop a method for microextraction and quantification of long and medium chain fatty acids from milk. The proposed method was carried out on three steps: (1) lipids extraction from 10μL of whole or skimmed milk containing omega-3 fatty acids (FAs) during 12, 24, 48, 72 and 96h, in 2mL chloroform: methanol mixture (2:1); (2) acid methylation of FAs; and (3) quantification of FAs methyl esters by gas chromatography. Using this method, the percentage recovery of FAs was higher (67% for whole and 85% for skimmed milk) compared with AOAC method 905.02 recovery (49% and 83%, respectively). Good reproducibility and repeatability (<3%) were obtained. The method developed can be useful for researching and in routine quality control. Additionally, it is simpler, faster and cheaper than the reference procedure since it requires minimum sample and solvents volume as well as fewer steps.