Senior Thai Fecal Microbiota Comparison Between Vegetarians and Non-Vegetarians Using PCR-DGGE and Real-Time PCR

• Published in: Journal of microbiology and biotechnology Vol. 24; no. 8; pp. 1026 - 1033
• Main Authors: Ruengsomwong, Supatjaree, Korenori, Yuki, Sakamoto, Naoshige, Wannissorn, Bhusita, Nakayama, Jiro, Nitisinprasert, Sunee
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.08.2014
• Subjects: Asian Continental Ancestry Group; Bacteria - classification; Bacteria - genetics; Biota; Denaturing Gradient Gel Electrophoresis; Diet, Vegetarian; Feces - microbiology; Humans; Real-Time Polymerase Chain Reaction; 생물학

The fecal microbiotas were investigated in 13 healthy Thai subjects using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). Among the 186 DNA bands detected on the polyacrylamide gel, 37 bands were identified as representing 11 species: Bacteroides thetaiotaomicron, Bacte...