Immunological methods for the detection of porcine lymphotropic herpesviruses (PLHV)

• Published in: Journal of virological methods Vol. 233; pp. 72 - 77
• Main Authors: Plotzki, Elena, Keller, Martina, Ehlers, Bernhard, Denner, Joachim
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2016
• Subjects: Animals; antibodies; Antibodies, Viral; Blotting, Western; bone marrow transplant; Butcher; ELISA; Enzyme-Linked Immunosorbent Assay; Epstein-Barr virus; Gene Expression; glycoproteins; Herpesviridae - genetics; Herpesviridae - immunology; Herpesviridae Infections - diagnosis; Herpesviridae Infections - immunology; Herpesviridae Infections - virology; Human gammaherpesvirus 4; Human gammaherpesvirus 8; Human herpesvirus 4; Human herpesvirus 8; Humans; lymphatic diseases; miniature swine; Pig; Polymerase Chain Reaction; Porcine lymphotropic herpesvirus; Recombinant Proteins; Sensitivity and Specificity; signs and symptoms (animals and humans); slaughterhouses; Swine; Swine Diseases - diagnosis; Swine Diseases - immunology; Swine Diseases - virology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Envelope Proteins - isolation & purification; Viral Envelope Proteins - metabolism; Western blot; Western blotting; Xenotransplantation; Western blot; Butcher; Porcine lymphotropic herpesvirus; Xenotransplantation; ELISA; Pig

•Development of an ELISA and a new Western blot assay to test PHLV.•Testing of different pigs, some of them were antibody positive.•No anti-PLHV antibodies were found in butchers. Porcine lymphotropic herpesviruses (PLHV-1, -2, and -3) are widespread in pigs and closely related to the human pathogen...