Protocol for RNA extraction from glioblastoma cell-line-derived spheroids embedded in atelocollagen gel guided by real-time imaging

• Published in: STAR protocols Vol. 6; no. 2; p. 103839
• Main Authors: Fujita, Mayumi, Imadome, Kaori, Sato, Tetsuro, Hirakawa, Hirokazu, Kado, Junko, Yamagiri, Asako, Miura, Taichi, Kamimura, Satoshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.06.2025; Elsevier
• Subjects: Cancer; Cell culture; Cell Line, Tumor; Collagen - chemistry; Gels - chemistry; Gene Expression Profiling - methods; Glioblastoma - genetics; Glioblastoma - pathology; Humans; Organoids; Protocol; RNA - isolation & purification; Spheroids, Cellular - chemistry; Spheroids, Cellular - metabolism; Organoids; Cell culture; Cancer

Microscopy advancements enable real-time imaging and analysis of cancer spheroids embedded in atelocollagen gel. Here, we present a protocol for extracting high-quality total RNA from 3D tumor spheroids embedded in atelocollagen gel, including spheroids and invading cells dispersed into surrounding...