Spatial Vulnerabilities of the Escherichia coli Genome to Spontaneous Mutations Revealed with Improved Duplex Sequencing

• Published in: Genetics (Austin) Vol. 210; no. 2; pp. 547 - 558
• Main Authors: Zhang, Xiaolong, Zhang, Xuehong, Zhang, Xia, Liao, Yuwei, Song, Luyao, Zhang, Qingzheng, Li, Peiying, Tian, Jichao, Shao, Yanyan, Ai-Dherasi, Aisha Mohammed, Li, Yulong, Liu, Ruimei, Chen, Tao, Deng, Xiaodi, Zhang, Yu, Lv, Dekang, Zhao, Jie, Chen, Jun, Li, Zhiguang
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.10.2018
• Subjects: Annotations; Bacteria; Bioinformatics; Cell culture; Deoxyribonucleic acid; DNA; DNA damage; Domains; E coli; Escherichia coli; Escherichia coli - genetics; Evolution; Gene sequencing; Genetic Loci; Genetics; Genome, Bacterial; Genomes; Investigations; Models, Genetic; Mutation; Mutation Rate; Next-generation sequencing; Population; Sequence Analysis, DNA - methods; Very Low Frequencies; United States > US; spontaneous mutations; hotspot; duplex sequencing; Escherichia coli; binomial distribution

Investigation of spontaneous mutations by next-generation sequencing technology has attracted extensive attention lately due to the fundamental roles of spontaneous mutations in evolution and pathological processes. However, these studies only focused on the mutations accumulated through many genera...