Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme

• Published in: Nucleic acids research Vol. 29; no. 2; pp. E3 - 3
• Main Authors: Szatmari, I, Aradi, J
• Format: Journal Article
• Language: English
• Published: England Oxford Publishing Limited (England) 15.01.2001; Oxford University Press
• Subjects: Cell Line; DNA Primers - chemical synthesis; Gene Amplification; HeLa Cells; HL-60 Cells; Humans; NAR Methods Online; Nucleic Acid Amplification Techniques; Polymerase Chain Reaction - methods; Protein Processing, Post-Translational; Repetitive Sequences, Nucleic Acid; Substrate Specificity; Telomerase - genetics; Telomerase - metabolism; Telomere - enzymology; Telomere - genetics; Telomeric Repeat Amplification Protocol; TRAP; Tumor Cells, Cultured

The Telomeric Repeat Amplification Protocol (TRAP) and its modified versions (including ours, TP-TRAP) change the size and/or the ratio of the telomerase products in the amplification stage of the assay. Based on our recently published method we developed a new TRAP. This method ensures that the num...