Local Somatic Hypermutation and Class Switch Recombination in the Nasal Mucosa of Allergic Rhinitis Patients

• Published in: The Journal of immunology (1950) Vol. 171; no. 10; pp. 5602 - 5610
• Main Authors: Coker, Heather A, Durham, Stephen R, Gould, Hannah J
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15.11.2003
• Subjects: Adolescent; Adult; Amino Acid Sequence; B-Lymphocyte Subsets - enzymology; B-Lymphocyte Subsets - immunology; B-Lymphocyte Subsets - metabolism; Base Sequence; Clone Cells; Cytidine Deaminase - biosynthesis; Cytidine Deaminase - genetics; Humans; Immunoglobulin A - biosynthesis; Immunoglobulin Class Switching - genetics; Immunoglobulin Constant Regions - biosynthesis; Immunoglobulin Constant Regions - genetics; Immunoglobulin E - biosynthesis; Immunoglobulin Heavy Chains - biosynthesis; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Variable Region - biosynthesis; Immunoglobulin Variable Region - genetics; Lymphocyte Activation - genetics; Male; Middle Aged; Molecular Sequence Data; Multigene Family - immunology; Nasal Mucosa - immunology; Nasal Mucosa - metabolism; Recombination, Genetic - immunology; Rhinitis, Allergic, Perennial - enzymology; Rhinitis, Allergic, Perennial - genetics; Rhinitis, Allergic, Perennial - immunology; RNA, Messenger - biosynthesis; Sequence Analysis, DNA; Somatic Hypermutation, Immunoglobulin

Immunoglobulin E is produced by nasal B cells in response to allergen. We have analyzed IgE VH region sequences expressed in the nasal mucosa of patients suffering from allergic rhinitis. VH region sequences were amplified by RT-PCR from IgE+ B cells from nasal biopsies. In two of six patients, sequ...