In situ cryo-electron tomography: a new method to elucidate cytoplasmic zoning at the molecular level

• Published in: Journal of biochemistry (Tokyo) Vol. 175; no. 2; pp. 187 - 193
• Main Authors: Chen, Lin, Fukata, Yuko, Murata, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 25.02.2024
• Subjects: Aqueous solutions; Biomolecules; Electron microscopy; Ion beams; Liquid phases; Phase separation; Scanning electron microscopy; Structural analysis; Tomography; Zoning; cryo-electron tomography; liquid–liquid phase separation; cryo-focused ion beam scanning electron microscope; cytoplasmic zoning; cryo-electron microscopy; liquid-liquid phase separation

Abstract Cryo-electron microscopy was developed as a powerful tool for imaging biological specimens in near-native conditions. Nowadays, advances in technology, equipment and computations make it possible to obtain structures of biomolecules with near-atomic resolution. Furthermore, cryo-electron to...