A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis

• Published in: Cell Vol. 163; no. 2; pp. 445 - 455
• Main Authors: Zhai, Jixian, Bischof, Sylvain, Wang, Haifeng, Feng, Suhua, Lee, Tzuu-fen, Teng, Chong, Chen, Xinyuan, Park, Soo Young, Liu, Linshan, Gallego-Bartolome, Javier, Liu, Wanlu, Henderson, Ian R., Meyers, Blake C., Ausin, Israel, Jacobsen, Steven E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08.10.2015
• Subjects: Adenine - metabolism; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; biogenesis; DNA; DNA Methylation; DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - metabolism; Models, Biological; nucleotides; RNA, Plant - genetics; RNA, Small Interfering - genetics; small interfering RNA; Transcription Initiation Site

RNA-directed DNA methylation in Arabidopsis thaliana is driven by the plant-specific RNA Polymerase IV (Pol IV). It has been assumed that a Pol IV transcript can give rise to multiple 24-nt small interfering RNAs (siRNAs) that target DNA methylation. Here, we demonstrate that Pol IV-dependent RNAs (...