Molecular Cloning of Syndecan, an Integral Membrane Proteoglycan

• Published in: The Journal of cell biology Vol. 108; no. 4; pp. 1547 - 1556
• Main Authors: Saunders, Scott, Jalkanen, Markku, O'Farrell, Susan, Bernfield, Merton
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.04.1989; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Amino acids; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Binding Sites; Biological and medical sciences; Blotting, Northern; Cell Line; Cell Membrane - metabolism; Cells; Chondroitin Sulfates - metabolism; Cloning, Molecular; Complementary DNA; DNA - genetics; Epithelial cells; Female; Fundamental and applied biological sciences. Psychology; Glycoproteins; Heparitin Sulfate - metabolism; Integrins; Mammary Glands, Animal; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Messenger RNA; Mice; Molecular Sequence Data; Proteins; Proteoglycans; Receptors; Restriction Mapping; RNA; Sequence Homology, Nucleic Acid; Sulfates; Syndecans; Proteoglycan; Membrane protein; Intrinsic protein; Rodentia; Glycoproteins; Gene library; Cell surface; Northern blotting; Vertebrata; Mammalia; Complementary DNA; Mouse; Plasma membrane; Mammary gland; Molecular cloning; Aminoacid sequence

We describe cDNA clones for a cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. The cDNA encodes a unique core protein of 32,868 D that contains several structural features consistent with its role as a glyco...