In silico identification and characterization of putative differentially expressed genes involved in common bean (Phaseolus vulgaris L.) seed development

• Published in: Plant cell, tissue and organ culture Vol. 107; no. 2; pp. 341 - 353
• Main Authors: Abid, Ghassen, Muhovski, Yordan, Jacquemin, Jean-Marie, Mingeot, Dominique, Sassi, Khaled, Toussaint, André, Baudoin, Jean-Pierre
• Format: Journal Article Web Resource
• Language: English
• Published: Dordrecht Springer Netherlands 01.11.2011; Springer; Springer Nature B.V; Springer Science & Business Media B.V
• Subjects: Abnormalities; Abortion; Agriculture & agronomie; Agriculture & agronomy; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cotyledons; Cultivars; Developmental stages; Disruption; Embryogenesis; Embryonic growth stage; Embryos; EMS mutant; Endosperm; ESTs; Ethyl methanesulfonate; Feasibility studies; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Genotypes; Homology; Life Sciences; Original Paper; Phaseolus vulgaris; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; RT-PCR; Sciences du vivant; Seed coats; Seed development; Seeds; EMS mutant; RT–PCR; Seed development; ESTs; Vegetals; Seeds; Identification; Gene expression; Bean; Characterization; Leguminosae; Phaseolus vulgaris; Dicotyledones; Seed; RT-PCR; Angiospermae; Development; Spermatophyta; Mutation; Reverse transcription polymerase chain reaction; Computer aided analysis
• ISSN: 0167-6857; 1573-5044; 1573-5044
• DOI: 10.1007/s11240-011-9986-7

Two genotypes of common bean ( Phaseolus vulgaris L.) were studied to determine the structural cause of seed abortion in this species. In the non-abortive control (wild-type, cultivar BAT93), the histological analysis revealed a classical pattern of seed development and showed coordinated differentiation of the embryo proper, suspensor, endosperm tissue and seed coat. In contrast, the ethyl methanesulfonate (EMS) mutant (cultivar BAT93) showed disruption in the normal seed development leading to embryo abortion. Aborted embryos from these degenerate seeds showed abnormalities in suspensor and cotyledons at the globular, heart, torpedo and cotyledon stages. Exploring the feasibility of incorporating the available online bioinformatics databases, we identified 22 genes revealing high homology with genes involved in Arabidopsis thaliana embryo development and expressed in common bean immature seeds. The expression patterns of these genes were confirmed by RT–PCR. All genes were highly expressed in seed tissues. To study the expression profiles of isolated genes during Phaseolus embryogenesis, six selected genes were examined by quantitative RT–PCR analysis on the developing embryos of wild-type and EMS mutant plants. All selected genes were expressed differentially at different stages of embryo development. These results could help to improve understanding of the mechanism of common bean embryogenesis.