reduction in ATP demand and mitochondrial activity with neural differentiation of human embryonic stem cells

• Published in: Journal of cell science Vol. 124; no. 3; pp. 348 - 358
• Main Authors: Birket, Matthew J, Orr, Adam L, Gerencser, Akos A, Madden, David T, Vitelli, Cathy, Swistowski, Andrzej, Brand, Martin D, Zeng, Xianmin
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Limited 01.02.2011; Company of Biologists
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Adenosine Triphosphate - metabolism; Carrier Proteins - metabolism; Cell Differentiation; Cell Proliferation; Culture Media, Conditioned; Embryonic Stem Cells - metabolism; Embryonic Stem Cells - ultrastructure; Energy Metabolism; Heat-Shock Proteins - metabolism; Humans; Mitochondria - metabolism; Mitochondria - ultrastructure; Neural Stem Cells - metabolism; Neural Stem Cells - ultrastructure; Nuclear Proteins - metabolism; Oxidative Phosphorylation; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Transcription Factors - metabolism

Here, we have investigated mitochondrial biology and energy metabolism in human embryonic stem cells (hESCs) and hESC-derived neural stem cells (NSCs). Although stem cells collectively in vivo might be expected to rely primarily on anaerobic glycolysis for ATP supply, to minimise production of react...