Homology-dependent interactions determine the order of strand exchange by IntDOT recombinase

• Published in: Nucleic acids research Vol. 38; no. 3; pp. 958 - 969
• Main Authors: Laprise, Jennifer, Yoneji, Sumiko, Gardner, Jeffrey F
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2010
• Subjects: Attachment Sites, Microbiological; Bacteroides; Bacteroides - enzymology; DNA - chemistry; DNA - metabolism; DNA Cleavage; Integrases - metabolism; Nucleic Acid Enzymes; Sequence Homology, Nucleic Acid

The Bacteroides conjugative transposon CTnDOT encodes an integrase, IntDOT, which is a member of the tyrosine recombinase family. Other members of this group share a strict requirement for sequence identity within the region of strand exchange, called the overlap region. Tyrosine recombinases cataly...