Metallothionein expression is increased in monocytes and erythrocytes of young men during zinc supplementation

• Published in: The Journal of nutrition Vol. 128; no. 4; pp. 707 - 713
• Main Authors: Sullivan, V.K. (University of North Florida, Jacksonville, FL.), Burnett, F.R, Cousins, R.J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Nutritional Sciences 01.04.1998; American Institute of Nutrition
• Subjects: Adult; ADULTE; ADULTOS; ADULTS; ARN MENSAJERO; ARN MESSAGER; Biological and medical sciences; blood; BLOOD PLASMA; CINC; COBRE; COMPLEMENT ALIMENTAIRE; COPPER; Copper - blood; CUIVRE; dietary mineral supplements; Dietary Supplements; duration; Enzyme-Linked Immunosorbent Assay; ERITROCITOS; ERYTHROCYTE; ERYTHROCYTES; Erythrocytes - metabolism; EXPRESION GENICA; EXPRESSION DES GENES; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; genetics; HOMBRES; HOMME; Humans; Male; MEN; MESSENGER RNA; metabolism; METALLOPROTEINE; METALLOPROTEINS; metallothionein; Metallothionein - genetics; Metallothionein - metabolism; METALPROTEINAS; MINERAL NUTRIENTS; MONOCITOS; MONOCYTE; MONOCYTES; Monocytes - metabolism; NUTRIENTES MINERALES; pharmacology; PLASMA SANGUIN; PLASMA SANGUINEO; Polymerase Chain Reaction; Proteins; RNA, Messenger; RNA, Messenger - metabolism; SUBSTANCE NUTRITIVE MINERALE; SUPLEMENTOS; SUPPLEMENTS; TEMPS; THIONEINE; THIONEINS; TIEMPO; TIME; Time Factors; TIONEINA; Transcription, Genetic; Vertebrates: anatomy and physiology, studies on body, several organs or systems; YOUNG ADULTS; ZINC; Zinc - blood; Zinc - pharmacology; Micronutrient; Human; Blood cell; Monocyte; Red blood cell; Biological marker; Trace element (nutrient); Supplementation; Gene expression; Nutritional status; Metallothionein; Zinc

The metallothionein gene is transcriptionally regulated by zinc. Consequently, metallothionein has potential for serving as an index of dietary zinc status in humans. To examine this possibility, an enzyme-linked immunoassay (ELISA) based on a sandwich approach that utilizes monoclonal and chicken e...