Platelet-Collagen Adhesion: Evidence for Participation of Antigenically Distinct Entities

• Published in: The Journal of cell biology Vol. 99; no. 6; pp. 2048 - 2055
• Main Authors: Shadle, Paula J., Barondes, Samuel H.
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.12.1984; The Rockefeller University Press
• Subjects: Adhesion; Antibodies; Antigens; Antiserum; Biological and medical sciences; Blood coagulation. Blood cells; Blood Platelets - immunology; Blood Platelets - physiology; Cell adhesion; Collagen; Collagens; Epitopes - analysis; Factor VIII - analysis; Fibrinogen - analysis; Fibronectins - analysis; Fibrosis; Fundamental and applied biological sciences. Psychology; Gels; Humans; Immunoglobulin Fab Fragments; Molecular and cellular biology; Molecules; Platelet; Platelet Aggregation; Platelets; Human; Blood cell; Regulation(control); Platelet; Collagen; Adhesion; Immunological method
• DOI: 10.1083/jcb.99.6.2048
• ISSN: 0021-9525

Univalent antibody fragments prepared from a rabbit antiserum raised against whole human platelets completely inhibited adhesion of platelets to immobilized trimeric collagen in a defined, Mg2+-dependent, adhesion assay. An octylglucoside extract of whole platelets completely neutralized this antibody, and all neutralizing activity bound to immobilized wheat germ agglutinin. Further fractionation on concanavalin A gave rise to subfractions that each neutralized only partially at saturation, when tested against antibody concentrations that inhibit 50% of platelet-collagen adhesion. When tested against higher antibody concentrations that completely inhibited adhesion, each subfraction had no detectable neutralizing effect, although the combined subfractions neutralized completely. This and other evidence suggests that more than one platelet entity participates in platelet-collagen adhesion. Although distinct, they appear to play interdependent roles in a single adhesion process.