Novel Bacteriophage Specific against Staphylococcus epidermidis and with Antibiofilm Activity

has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are not life-threatening, their frequency and the fact that they are extremely difficult to treat represent a serious burden on the public health system. Treatment is complic...

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Published inViruses Vol. 14; no. 6; p. 1340
Main Authors Fanaei Pirlar, Rima, Wagemans, Jeroen, Ponce Benavente, Luis, Lavigne, Rob, Trampuz, Andrej, Gonzalez Moreno, Mercedes
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 20.06.2022
MDPI
Subjects
Adsorption
Anti-Bacterial Agents - therapeutic use
Antibiotic resistance
Antibiotics
Bacteria
Bacterial infections
Bacteriophages - genetics
biofilm-associated infection
Biofilms
Efficiency
Genomes
Host range
Humans
Infections
isothermal microcalorimetry
Lysis
Lysogeny
Medical equipment
novel bacteriophage species
Pathogens
pH effects
Phages
Polyethylene glycol
Public health
Sewage
Staphylococcal Infections - microbiology
Staphylococcus epidermidis
Structural proteins
Transmission electron microscopy
Transplants & implants
Virulence
whole-genome sequencing
United States > US
Germany
novel bacteriophage species
Staphylococcus epidermidis
biofilm-associated infection
whole-genome sequencing
isothermal microcalorimetry
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Abstract has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are not life-threatening, their frequency and the fact that they are extremely difficult to treat represent a serious burden on the public health system. Treatment is complicated by specific antibiotic resistance genes and the formation of biofilms. Hence, novel therapeutic strategies are needed to fight these infections. A novel bacteriophage CUB-EPI_14 specific to the bacterial species was isolated from sewage and characterized genomically and phenotypically. Its genome contains a total of 46,098 bp and 63 predicted genes, among which some have been associated with packaging and lysis-associated proteins, structural proteins, or DNA- and metabolism-associated proteins. No lysogeny-associated proteins or known virulence proteins were identified in the phage genome. CUB-EPI_14 showed stability over a wide range of temperatures (from -20 °C to 50 °C) and pH values (pH 3-pH 12) and a narrow host range against . Potent antimicrobial and antibiofilm activities were observed when the phage was tested against a highly susceptible bacterial isolate. These encouraging results open the door to new therapeutic opportunities in the fight against resilient biofilm-associated infections caused by .
AbstractList Staphylococcus epidermidis has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are not life-threatening, their frequency and the fact that they are extremely difficult to treat represent a serious burden on the public health system. Treatment is complicated by specific antibiotic resistance genes and the formation of biofilms. Hence, novel therapeutic strategies are needed to fight these infections. A novel bacteriophage CUB-EPI_14 specific to the bacterial species S. epidermidis was isolated from sewage and characterized genomically and phenotypically. Its genome contains a total of 46,098 bp and 63 predicted genes, among which some have been associated with packaging and lysis-associated proteins, structural proteins, or DNA- and metabolism-associated proteins. No lysogeny-associated proteins or known virulence proteins were identified in the phage genome. CUB-EPI_14 showed stability over a wide range of temperatures (from −20 °C to 50 °C) and pH values (pH 3–pH 12) and a narrow host range against S. epidermidis . Potent antimicrobial and antibiofilm activities were observed when the phage was tested against a highly susceptible bacterial isolate. These encouraging results open the door to new therapeutic opportunities in the fight against resilient biofilm-associated infections caused by S. epidermidis .
has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are not life-threatening, their frequency and the fact that they are extremely difficult to treat represent a serious burden on the public health system. Treatment is complicated by specific antibiotic resistance genes and the formation of biofilms. Hence, novel therapeutic strategies are needed to fight these infections. A novel bacteriophage CUB-EPI_14 specific to the bacterial species was isolated from sewage and characterized genomically and phenotypically. Its genome contains a total of 46,098 bp and 63 predicted genes, among which some have been associated with packaging and lysis-associated proteins, structural proteins, or DNA- and metabolism-associated proteins. No lysogeny-associated proteins or known virulence proteins were identified in the phage genome. CUB-EPI_14 showed stability over a wide range of temperatures (from -20 °C to 50 °C) and pH values (pH 3-pH 12) and a narrow host range against . Potent antimicrobial and antibiofilm activities were observed when the phage was tested against a highly susceptible bacterial isolate. These encouraging results open the door to new therapeutic opportunities in the fight against resilient biofilm-associated infections caused by .
Staphylococcus epidermidis has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are not life-threatening, their frequency and the fact that they are extremely difficult to treat represent a serious burden on the public health system. Treatment is complicated by specific antibiotic resistance genes and the formation of biofilms. Hence, novel therapeutic strategies are needed to fight these infections. A novel bacteriophage CUB-EPI_14 specific to the bacterial species S. epidermidis was isolated from sewage and characterized genomically and phenotypically. Its genome contains a total of 46,098 bp and 63 predicted genes, among which some have been associated with packaging and lysis-associated proteins, structural proteins, or DNA- and metabolism-associated proteins. No lysogeny-associated proteins or known virulence proteins were identified in the phage genome. CUB-EPI_14 showed stability over a wide range of temperatures (from −20 °C to 50 °C) and pH values (pH 3–pH 12) and a narrow host range against S. epidermidis. Potent antimicrobial and antibiofilm activities were observed when the phage was tested against a highly susceptible bacterial isolate. These encouraging results open the door to new therapeutic opportunities in the fight against resilient biofilm-associated infections caused by S. epidermidis.
Author Fanaei Pirlar, Rima
Trampuz, Andrej
Gonzalez Moreno, Mercedes
Wagemans, Jeroen
Lavigne, Rob
Ponce Benavente, Luis
AuthorAffiliation 1 Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Center for Musculoskeletal Surgery, Augustenburger Platz 1, 13353 Berlin, Germany; rima.fanaei@charite.de (R.F.P.); luis.ponce-benavente@charite.de (L.P.B.); andrej.trampuz@charite.de (A.T.)
2 Berlin Institute of Health at Charité, Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Charitéplatz 1, 10117 Berlin, Germany
3 KU Leuven, Department of Biosystems, Kasteelpark Arenberg 21, 3001 Leuven, Belgium; jeroen.wagemans@kuleuven.be (J.W.); rob.lavigne@kuleuven.be (R.L.)
AuthorAffiliation_xml – name: 1 Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Center for Musculoskeletal Surgery, Augustenburger Platz 1, 13353 Berlin, Germany; rima.fanaei@charite.de (R.F.P.); luis.ponce-benavente@charite.de (L.P.B.); andrej.trampuz@charite.de (A.T.)
– name: 2 Berlin Institute of Health at Charité, Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Charitéplatz 1, 10117 Berlin, Germany
– name: 3 KU Leuven, Department of Biosystems, Kasteelpark Arenberg 21, 3001 Leuven, Belgium; jeroen.wagemans@kuleuven.be (J.W.); rob.lavigne@kuleuven.be (R.L.)
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  surname: Gonzalez Moreno
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Issue 6
Keywords novel bacteriophage species
Staphylococcus epidermidis
biofilm-associated infection
whole-genome sequencing
isothermal microcalorimetry
Language English
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SSID ssj0066907
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Snippet has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are not life-threatening, their...
Staphylococcus epidermidis has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are...
Staphylococcus epidermidis has emerged as the most important pathogen in infections related to indwelling medical devices, and although these infections are...
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SubjectTerms Adsorption
Anti-Bacterial Agents - therapeutic use
Antibiotic resistance
Antibiotics
Bacteria
Bacterial infections
Bacteriophages - genetics
biofilm-associated infection
Biofilms
Efficiency
Genomes
Host range
Humans
Infections
isothermal microcalorimetry
Lysis
Lysogeny
Medical equipment
novel bacteriophage species
Pathogens
pH effects
Phages
Polyethylene glycol
Public health
Sewage
Staphylococcal Infections - microbiology
Staphylococcus epidermidis
Structural proteins
Transmission electron microscopy
Transplants & implants
Virulence
whole-genome sequencing
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Title Novel Bacteriophage Specific against Staphylococcus epidermidis and with Antibiofilm Activity
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Volume 14
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