Use of a 3T3 cell growth factor assay for the delineation and characterization of humoral trophic factors involved in intestinal adaptation in the rat

• Published in: Gastroenterology (New York, N.Y. 1943) Vol. 91; no. 2; p. 448
• Main Authors: Biller, J A, Montgomery, R K, Grand, R J, Klagsbrun, M, Rosenthal, A
• Format: Journal Article
• Language: English
• Published: United States 01.08.1986
• Subjects: Adaptation, Physiological; Animals; Autoradiography; Biological Assay - methods; Cell Line; Dialysis; DNA - biosynthesis; Fibroblasts; Growth Substances - analysis; Ileum - metabolism; Intestinal Mucosa - metabolism; Intestines - physiology; Intestines - surgery; Male; Molecular Weight; Rats; Rats, Inbred Strains; Scintillation Counting; Time Factors

Indirect evidence has suggested that circulating trophic factors are involved in intestinal adaptation. A 3T3 fibroblast cell culture system was used to more directly delineate the presence of such factors. Rats were divided into four groups: C-unop, those undergoing no surgery; S, those in which a...